CAJ | 학술논문

目的：研究干扰素诱导的跨膜蛋白5(IFITM5)在人骨肉瘤15种细胞系中的表达。方法应用RT-QPCR和Western blot检测IFITM5在SAOS2、3A0、SKOV3、HEPG2、HEP2、ACCM、HT29、MDA-MB-231、H7402、HELA、HEK-293、HL-60、K562、THP1、RD等癌细胞系中mRNA和蛋白表达差异。结果在H7402、RD、HEP2细胞系中IFITM5 mRNA和蛋白的表达水平最低，其他细胞系次之，在SAOS2和3A0细胞系中两者均呈高表达。结论 IFITM5在癌细胞系中存在并有表达差异，为后续研究IFITM5在肿瘤中的作用奠定基础。
목적：연구간우소유도적과막단백5(IFITM5)재인골육류15충세포계중적표체。방법응용RT-QPCR화Western blot검측IFITM5재SAOS2、3A0、SKOV3、HEPG2、HEP2、ACCM、HT29、MDA-MB-231、H7402、HELA、HEK-293、HL-60、K562、THP1、RD등암세포계중mRNA화단백표체차이。결과재H7402、RD、HEP2세포계중IFITM5 mRNA화단백적표체수평최저，기타세포계차지，재SAOS2화3A0세포계중량자균정고표체。결론 IFITM5재암세포계중존재병유표체차이，위후속연구IFITM5재종류중적작용전정기출。
Objective To investigate the expression level of IFN-inducible transmembrane protein 5(IFITM5) in 15 different cancer cell lines.Methods RT-QPCR and Western blot were performed to detect the expression level of IFITM5 in SAOS2,3A0, SKOV3, HEPG2, HEP2, ACCM, HT29, MDA-MB-231, H7402, HELA, HEK-293, HL-60, K562, THP1 and RD cancer cell lines.Results There was remarkable difference in the expression of IFITM5 in this cancer cell lines.The lowest expression level of mRNA and protein were detected in H7402,RD and HEP2 cell lines .The highest IFITM5 expression was observed in SAOS2 cells,followed by 3AO.Conclusions Signiifcant differential expression of IFITM5 in 15 cancer cells was observed, which laid the foundation for further research on the relationship of IFITM5 and tumor.