CAJ | 학술논문

以罗丹明6G和牛血清白蛋白的复合物为荧光探针，银纳米三角片为猝灭剂，研究了银纳米三角片与荧光复合物的荧光共振能量转移现象，建立了测定钴离子的荧光分析法。研究发现，一定浓度的荧光复合物与银纳米三角片混合后，由于荧光复合物在银纳米三角片上吸附而发生荧光共振能量转移，荧光猝灭达到80％左右。当钴离子存在时，银纳米三角片与罗丹明6G荧光共振能量转移被破坏，荧光逐渐恢复。随着钴离子浓度的增加，体系荧光值的恢复率（ I／I0）与钴离子的浓度（cCo2＋）有良好的线性关系，线性回归方程为 I／I0＝1.054＋21.72 cCo2＋，相关系数 r2为0.9962。通过对自然水样进行加标回收检测，实现了钴离子的定量检测，回收率在90.4％～115.1％之间。建立了一种可靠的选择性检测钴离子的荧光分析方法。
이라단명6G화우혈청백단백적복합물위형광탐침，은납미삼각편위졸멸제，연구료은납미삼각편여형광복합물적형광공진능량전이현상，건립료측정고리자적형광분석법。연구발현，일정농도적형광복합물여은납미삼각편혼합후，유우형광복합물재은납미삼각편상흡부이발생형광공진능량전이，형광졸멸체도80％좌우。당고리자존재시，은납미삼각편여라단명6G형광공진능량전이피파배，형광축점회복。수착고리자농도적증가，체계형광치적회복솔（ I／I0）여고리자적농도（cCo2＋）유량호적선성관계，선성회귀방정위 I／I0＝1.054＋21.72 cCo2＋，상관계수 r2위0.9962。통과대자연수양진행가표회수검측，실현료고리자적정량검측，회수솔재90.4％～115.1％지간。건립료일충가고적선택성검측고리자적형광분석방법。
In the present paper ,the authors studied fluorescence resonance energy transfer (FRET ) phenomenon between silver triangular nanoplates and bovine serum albumin (BSA )/Rhodamine 6G fluorescence complex ,and established a fluorescence method for the detection of cobalt ions .We found that when increasing the silver triangular nanoplates added to certain concen‐trations of fluorescent bovine serum albumin (BSA ) /Rhodamine 6G complex , the fluorescence of Rhodamine 6G would be quenched up to 80% due to the FRET between the quencher and donor .However ,in the presence of cobalt ions ,the disassoci‐ation of the fluorescent complex from silver triangular nanoplates occurred and the fluorescence of the Rhodamine 6G recovered . The recovery of fluorescence intensity rate (I/I0 ) has a good relationship with the cobalt ion concentration (cCO2+ ) added . Thus ,the authors developed a fluorescence method for the detection of cobalt ions based on the FRET of silver triangular nano‐plates and Rhodamine 6G .