CAJ | 학술논문

采用耐辐射奇球菌活体为生物吸附剂，进行了对水溶液中放射性核素铀的批量吸附实验。主要研究了溶液pH、初始铀浓度对吸附放射性核素铀效果的影响，并通过红外光谱（FTIR）和扫描电镜／能谱仪（SEM／EDS）等手段分析了其吸附机理。结果表明：在pH 5，c0＝100 mg · L －1时其吸附效果最佳，而最大饱和吸附量qmax为240 mgU · g －1。结合扫描电镜与能谱分析结果认为，耐辐射奇球菌表面附着了大量的含铀片状结晶物，并且其吸附过程主要以离子交换或表面络合的方式吸附铀。比较吸附铀前后菌体红外光谱图和红外分峰图发现，耐辐射奇球菌吸附铀后的整体谱形有了一定的变化，特别是蛋白质中酰胺基团、羟基、羧基以及磷酸基团等活性基团可能参与了吸附过程，并且在906 cm－1处出现了一个新的峰，此峰为 U O2＋2的伸缩振动峰。可见，利用具有抗辐射特性的耐辐射奇球菌为生物吸附剂，以期在放射性环境中对放射性核素铀的吸附具有一定的潜在途径。
채용내복사기구균활체위생물흡부제，진행료대수용액중방사성핵소유적비량흡부실험。주요연구료용액pH、초시유농도대흡부방사성핵소유효과적영향，병통과홍외광보（FTIR）화소묘전경／능보의（SEM／EDS）등수단분석료기흡부궤리。결과표명：재pH 5，c0＝100 mg · L －1시기흡부효과최가，이최대포화흡부량qmax위240 mgU · g －1。결합소묘전경여능보분석결과인위，내복사기구균표면부착료대량적함유편상결정물，병차기흡부과정주요이리자교환혹표면락합적방식흡부유。비교흡부유전후균체홍외광보도화홍외분봉도발현，내복사기구균흡부유후적정체보형유료일정적변화，특별시단백질중선알기단、간기、최기이급린산기단등활성기단가능삼여료흡부과정，병차재906 cm－1처출현료일개신적봉，차봉위 U O2＋2적신축진동봉。가견，이용구유항복사특성적내복사기구균위생물흡부제，이기재방사성배경중대방사성핵소유적흡부구유일정적잠재도경。
As a biological adsorbent ,Living deinococcus radiodurans was used for removing radionuclide uranium in the aqueous solution .The effect factors on biosorption of radionuclide uranium were researched in the present paper ,including solution pH values and initial uranium concentration .Meanwhile ,the biosorption mechanism was researched by the method of FTIR and SEM/EDS .The results show that the optimum conditions for biosorption are as follows :pH= 5 ,c0 = 100 mg · L -1 and the maximum biosorption capacity is up to 240 mgU · g -1 .According to the SEM results and EDXS analysis ,it is indicated that the cell surface is attached by lots of sheet uranium crystals ,and the main biosorpiton way of uranium is the ion exchange or surface complexation .Comparing FTIR spectra and FTIR fitting spectra before and after biosorption ,we can find that the whole spectra has a certain change ,particularly active groups (such as amide groups of the protein ,hydroxy ,carboxyl and phosphate group ) are involved in the biosorption process .Then ,there is a new peak at 906 cm -1 and it is a stretching vibration peak of UO2+2 .Ob‐viously ,it is possible that as an anti radiation microorganism ,deinococcus radiodurans could be used for removing radionuclide uranium in radiation environment .