CAJ | 학술논문

水在中红外波段的强吸收有时会遮蔽水溶液中溶质的特征峰，从而干扰溶质的红外谱带归属和分析。提出了一种新方法，控制水峰的吸收强度逐渐变化（强→弱→消失），从而得到高质量的红外光谱。利用单次ATR附件，通过先扫描空ATR晶体N次，经短暂暂停后，再继续扫描载有纯水（或参比溶液）的ATR晶体M次，这样累加得到的背景单光束谱（N＋M次扫描）中水的吸收强度就直接与扫描次数N和M 相关。选择足够大的扫描次数N和合适的扫描次数 M，就可以彻底扣除水峰的干扰。利用该方法，成功获得了K2 CO3溶液和牛血清白蛋白（BSA ）溶液的消除了水吸收峰的高质量红外光谱。实验结果证实新方法具有快速、高效等优点。文中也讨论了该方法的局限性。
수재중홍외파단적강흡수유시회차폐수용액중용질적특정봉，종이간우용질적홍외보대귀속화분석。제출료일충신방법，공제수봉적흡수강도축점변화（강→약→소실），종이득도고질량적홍외광보。이용단차ATR부건，통과선소묘공ATR정체N차，경단잠잠정후，재계속소묘재유순수（혹삼비용액）적ATR정체M차，저양루가득도적배경단광속보（N＋M차소묘）중수적흡수강도취직접여소묘차수N화M 상관。선택족구대적소묘차수N화합괄적소묘차수 M，취가이철저구제수봉적간우。이용해방법，성공획득료K2 CO3용액화우혈청백단백（BSA ）용액적소제료수흡수봉적고질량홍외광보。실험결과증실신방법구유쾌속、고효등우점。문중야토론료해방법적국한성。
Infrared spectral studies of aqueous solution have been severely limited by the strong water absorption .Usually ,it is very difficult to experimentally achieve perfect compensation between the amount of water that contributes to the sample and background spectra .In the present paper ,we introduced a new method to make the infrared bands of water disappear during the measurement of an infrared spectrum for aqueous solution .Both background spectrum of the empty ATR cell and that of the water on ATR cell are used in sequence .Qualified infrared spectra of 10% K2 CO3 and 10% BSA aqueous solutions were success‐fully obtained by ATR accessory (single‐reflection) without water interference .The experimental results demonstrated that the new method is fast ,effective and powerful .The limitation of the new method is also discussed .