中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2015年
2期
111-114
,共4页
鼻咽肿瘤%疱疹病毒4型,人%抗体,病毒
鼻嚥腫瘤%皰疹病毒4型,人%抗體,病毒
비인종류%포진병독4형,인%항체,병독
Nasopharyngeal neoplasms%Herpesvirus 4,human%Antibodies,viral
目的 探讨EB病毒Rta蛋白IgG抗体(Rta-IgG)、EB病毒衣壳抗原IgA抗体(VCA-IgA)和EB病毒早期抗原IgA抗体(EA-IgA)在鼻咽癌筛查中的意义.方法 用调查法收集2012年5月至2013年7月在福建医科大学附属协和医院体检的健康人群体检组8 884名,因鼻咽部临床相关症状就诊我院耳鼻喉科的临床筛查组1 546例,经我院病理确诊为鼻咽癌的鼻咽癌组155例,3组血清标本的EB病毒Rta-IgG、VCA-IgA和EA-IgA用ELISA法检测的结果,并分析评价各指标在诊断鼻咽癌中的价值.3种抗体检出阳性率的比较用卡方检验.结果 在鼻咽癌组,EB病毒Rta-IgG、VCA-IgA和EA-IgA阳性检出率分别为81.9％(127/155) 、90.3％(140/155) 、48.3％ (75/155),与临床筛查组及体检组相比明显升高,且差异均有统计学意义(x2分别为1 538.6、479.3、643.3,P均＜0.01).VCA-IgA敏感度最高90.3％(140/155),EA-IgA特异度最好95.1(9 915/10 430);进一步分析显示,Rta-IgG、VCA-IgA和EA-IgA对鼻咽癌的诊断均有很好的阴性预测值,阴性预测值分别为99.7％(9 826/9 854)、99.8 ％(8 168/8 183)、99.2％ (9 915/9 995);联合检测Rta-IgG、VCA-IgA和EA-IgA有利于提高鼻咽癌诊断的敏感度94.1％ (146/155)和特异度98.9％(10 469/10 585).结论 鼻咽癌患者血清Rta-IgG、VCA-IgA、EA-IgA抗体均具有一定的临床诊断价值,联合检测这3项指标有助于提高鼻咽癌的诊断效率及准确性.
目的 探討EB病毒Rta蛋白IgG抗體(Rta-IgG)、EB病毒衣殼抗原IgA抗體(VCA-IgA)和EB病毒早期抗原IgA抗體(EA-IgA)在鼻嚥癌篩查中的意義.方法 用調查法收集2012年5月至2013年7月在福建醫科大學附屬協和醫院體檢的健康人群體檢組8 884名,因鼻嚥部臨床相關癥狀就診我院耳鼻喉科的臨床篩查組1 546例,經我院病理確診為鼻嚥癌的鼻嚥癌組155例,3組血清標本的EB病毒Rta-IgG、VCA-IgA和EA-IgA用ELISA法檢測的結果,併分析評價各指標在診斷鼻嚥癌中的價值.3種抗體檢齣暘性率的比較用卡方檢驗.結果 在鼻嚥癌組,EB病毒Rta-IgG、VCA-IgA和EA-IgA暘性檢齣率分彆為81.9％(127/155) 、90.3％(140/155) 、48.3％ (75/155),與臨床篩查組及體檢組相比明顯升高,且差異均有統計學意義(x2分彆為1 538.6、479.3、643.3,P均＜0.01).VCA-IgA敏感度最高90.3％(140/155),EA-IgA特異度最好95.1(9 915/10 430);進一步分析顯示,Rta-IgG、VCA-IgA和EA-IgA對鼻嚥癌的診斷均有很好的陰性預測值,陰性預測值分彆為99.7％(9 826/9 854)、99.8 ％(8 168/8 183)、99.2％ (9 915/9 995);聯閤檢測Rta-IgG、VCA-IgA和EA-IgA有利于提高鼻嚥癌診斷的敏感度94.1％ (146/155)和特異度98.9％(10 469/10 585).結論 鼻嚥癌患者血清Rta-IgG、VCA-IgA、EA-IgA抗體均具有一定的臨床診斷價值,聯閤檢測這3項指標有助于提高鼻嚥癌的診斷效率及準確性.
목적 탐토EB병독Rta단백IgG항체(Rta-IgG)、EB병독의각항원IgA항체(VCA-IgA)화EB병독조기항원IgA항체(EA-IgA)재비인암사사중적의의.방법 용조사법수집2012년5월지2013년7월재복건의과대학부속협화의원체검적건강인군체검조8 884명,인비인부림상상관증상취진아원이비후과적림상사사조1 546례,경아원병리학진위비인암적비인암조155례,3조혈청표본적EB병독Rta-IgG、VCA-IgA화EA-IgA용ELISA법검측적결과,병분석평개각지표재진단비인암중적개치.3충항체검출양성솔적비교용잡방검험.결과 재비인암조,EB병독Rta-IgG、VCA-IgA화EA-IgA양성검출솔분별위81.9％(127/155) 、90.3％(140/155) 、48.3％ (75/155),여림상사사조급체검조상비명현승고,차차이균유통계학의의(x2분별위1 538.6、479.3、643.3,P균＜0.01).VCA-IgA민감도최고90.3％(140/155),EA-IgA특이도최호95.1(9 915/10 430);진일보분석현시,Rta-IgG、VCA-IgA화EA-IgA대비인암적진단균유흔호적음성예측치,음성예측치분별위99.7％(9 826/9 854)、99.8 ％(8 168/8 183)、99.2％ (9 915/9 995);연합검측Rta-IgG、VCA-IgA화EA-IgA유리우제고비인암진단적민감도94.1％ (146/155)화특이도98.9％(10 469/10 585).결론 비인암환자혈청Rta-IgG、VCA-IgA、EA-IgA항체균구유일정적림상진단개치,연합검측저3항지표유조우제고비인암적진단효솔급준학성.
Objective To evaluate the value of Epstein-Barr virus (EBV) IgG antibody to EBV Rta protein(Rta-IgG),IgA antibody to EBV early antigen (EA-IgA) and IgA antibody to EBV viral capsid antigen(VCA-IgA) for nasopharyngeal carcinoma (NPC) diagnosis.Methods From May 2012 to July 2013,serum samples from 8 884 healthy donors,1 546 clinical screening patients and 155 NPC patients in Fujian Medical University Union Hospital were collected,and EBV Rta-IgG,EA-IgA and VCA-IgA were detected by ELISA.The ROC curve analysis and correlation analysis were performed to assess the value of Rta-IgG,EA-IgA and VCA-IgA for NPC diagnosis.The positive rate among three kinds of antibodies were compared with chi-square test.Results Positive rates of EBV Rta-IgG,VCA-IgA and EA-IgA in NPC patient group were 81.9％ (127/155),90.3％ (140/155) and 48.3％ (75/155),respectively,which were higher than those in clinical screening patient group and healthy donor group (x2 =1 538.6,479.3 and 643.3 respectively,P ＜ 0.01).The sensitivity of VCA-IgA (90.3％,140/155) and the specificity of EA-IgA (95.1％,9 915/10 430) were the highest in all groups.Further analysis showed that negative predictive value of EBV Rta-IgG,VCA-IgA and EA-IgA for NPC diagnosis were 99.7％ (9 826/9 854),99.8％ (8 168/8 183),99.2％ (9 915/9 995),respectively,suggesting that three anti-EBV antibodies showed very good negative predictive value for NPC diagnosis.Next,combined detection of three anti-EBV antibodies could improve the sensitivity (94.1％,146/155) and specificity (98.9％,10 469/10 585) for the NPC diagnosis.Conclusion The EBV Rta-IgG,VCA-IgA and EA-IgA all show the clinical value for NPC diagnosis,and combined detection for Rta-IgG,VCA-IgA and EA-IgA is more suitable to screen NPC and can improve the sensitivity and specificity of NPC diagnosis.
