CAJ | 학술논문

目的观察不同剂量X射线照射对小鼠辅助性T细胞相关细胞因子的影响.方法 100只BALB /c小鼠用随机数字表法分为4组,包括健康对照组和X射线照射组(分别予2、4和6 Gy照射),每组25只.于照射前、照射后7、14、21和28 d,对小鼠一般情况进行观察,对外周血进行白细胞计数,用蛋白芯片检测血清中辅助性T细胞相关细胞因子含量,并用ELISA确证细胞因子的浓度变化.结果照射后,小鼠外周血白细胞14d降至最低,同一时间点的下降程度随照射剂量增加而增加(F=86.267,P＜0.05).芯片法初筛提示,细胞因子IFN-IFTGF-β、IL-6、IL-17上调,IL-5、IL-23、TNF-α下调.ELISA检测可见,IL-17浓度随辐射剂量增高而明显增高,6 Gy组在7、14和21 d均明显高于对照(t=23.743、21.759和17.662,P＜0.05);IFN-γ/IL-4浓度在2和4 Gy照射后无明显变化,6 Gy照射后在7、14、21和28 d均比健康对照组明显增高(t=8.335、9.982、6.990和3.074,P＜0.05),呈先升高后降低的变化趋势,14 d达高峰.结论低于致死剂量的X射线照射可使小鼠血清IFN-γ 、TGF-β、IL-6、IL-17细胞因子浓度升高,呈Thl细胞偏移.
목적 관찰불동제량X사선조사대소서보조성T세포상관세포인자적영향.방법 100지BALB /c소서용수궤수자표법분위4조,포괄건강대조조화X사선조사조(분별여2、4화6 Gy조사),매조25지.우조사전、조사후7、14、21화28 d,대소서일반정황진행관찰,대외주혈진행백세포계수,용단백심편검측혈청중보조성T세포상관세포인자함량,병용ELISA학증세포인자적농도변화.결과 조사후,소서외주혈백세포14d강지최저,동일시간점적하강정도수조사제량증가이증가(F=86.267,P＜0.05).심편법초사제시,세포인자IFN-IFTGF-β、IL-6、IL-17상조,IL-5、IL-23、TNF-α하조.ELISA검측가견,IL-17농도수복사제량증고이명현증고,6 Gy조재7、14화21 d균명현고우대조(t=23.743、21.759화17.662,P＜0.05);IFN-γ/IL-4농도재2화4 Gy조사후무명현변화,6 Gy조사후재7、14、21화28 d균비건강대조조명현증고(t=8.335、9.982、6.990화3.074,P＜0.05),정선승고후강저적변화추세,14 d체고봉.결론 저우치사제량적X사선조사가사소서혈청IFN-γ 、TGF-β、IL-6、IL-17세포인자농도승고,정Thl세포편이.
Objective To study the effects of X-ray irradiation at different doses on the expressions of the T-helper cell related cytokines in mouse.Methods 100 BALB/c mice were randomly divided into four groups (25 mice in each) with 0,2,4 and 6 Gy X-ray irradiation.At 1,7,14,21 and 28 d after irradiation,the general situation of mice were observed,and the peripheral white blood cells were counted.The alterations of the serum helper T cell related cytokines were screened by protein chips and tested by ELISA.Results At 14 d after irradiation,the peripheral white blood cells of mice decreased to the lowest level.The number of peripheral white blood cells decrease along with the irradiation dose (Fdose =86.267,P ＜0.05).There were significant increases in the release of IFN-γ,TGF-β,L-6 and IL-17,but decreases in cytokine IL-5,IL-23 and TNF-α.ELISA analysis revealed that the increases of IL-17 correlated with the irradiation dose positively and the concentration of IL-17 in 6 Gy group were higher than control group at 1,7,14 and 21 d after irradiation (t =23.743,21.759 and 17.662,P ＜ 0.05).The ratio of IFN-γ/IL-4 significantly higher than the control group at 1,7,14,21 and 28 d after irradiation (t =8.335,9.982,6.990 and 3.074,P ＜ 0.05),and it approached to the highest level at 14 d and then began to decrease.Conclusions A low dose X-irradiation induces T-helper cell related cytokines IFN-γ and IL-17,and leads to Th1-biased immune responses in mice in vivo.