贵阳医学院学报
貴暘醫學院學報
귀양의학원학보
JOURNAL OF GUIYANG MEDICAL COLLEGE
2015年
3期
267-269
,共3页
子宫内膜异位症%基因,TNF-β G252A%基因频率%相关性%易感性%新生血管生成
子宮內膜異位癥%基因,TNF-β G252A%基因頻率%相關性%易感性%新生血管生成
자궁내막이위증%기인,TNF-β G252A%기인빈솔%상관성%역감성%신생혈관생성
endometriosis%gene,TNF-β G252A%gene frequency%relevance%susceptibility%angio-genesis
目的：探讨肿瘤坏死因子（ TNF-β）G252A基因在子宫内膜异位症发病机制中作用。方法：80例经腹腔镜和病理确诊的内异症（ Ems）患者为Ems组，80例同期需采用腹腔镜排除子宫内膜异位症的健康妇女为对照组，取两组妇女静脉血，采用限制性片段长度多态性聚合酶链反应（ PCR-RFLP）技术检测TNF-β G252A基因的基因频率，酶联免疫（ ELISA）法测定血清TNF-β的含量，分析子宫内膜异位症与TNFβ G252A基因之间的关系。结果：Ems组血清TNF-β的含量明显高于对照组（ P<0.05），且Ems组血清中TNF-β的含量与EmT的分期呈正相关（ r=0.8532）；Ems组的A/A 基因型及G/A 基因型频率均明显高于对照组，差异有统计学意义（ P<0.05）；A/A基因型患Ems的危险度是G/G基因型的3.5倍。结论：Ems患者血清中TNF-β的含量与EmT的分期呈正相关，A/A基因型是患Ems的危险基因型。
目的：探討腫瘤壞死因子（ TNF-β）G252A基因在子宮內膜異位癥髮病機製中作用。方法：80例經腹腔鏡和病理確診的內異癥（ Ems）患者為Ems組，80例同期需採用腹腔鏡排除子宮內膜異位癥的健康婦女為對照組，取兩組婦女靜脈血，採用限製性片段長度多態性聚閤酶鏈反應（ PCR-RFLP）技術檢測TNF-β G252A基因的基因頻率，酶聯免疫（ ELISA）法測定血清TNF-β的含量，分析子宮內膜異位癥與TNFβ G252A基因之間的關繫。結果：Ems組血清TNF-β的含量明顯高于對照組（ P<0.05），且Ems組血清中TNF-β的含量與EmT的分期呈正相關（ r=0.8532）；Ems組的A/A 基因型及G/A 基因型頻率均明顯高于對照組，差異有統計學意義（ P<0.05）；A/A基因型患Ems的危險度是G/G基因型的3.5倍。結論：Ems患者血清中TNF-β的含量與EmT的分期呈正相關，A/A基因型是患Ems的危險基因型。
목적：탐토종류배사인자（ TNF-β）G252A기인재자궁내막이위증발병궤제중작용。방법：80례경복강경화병리학진적내이증（ Ems）환자위Ems조，80례동기수채용복강경배제자궁내막이위증적건강부녀위대조조，취량조부녀정맥혈，채용한제성편단장도다태성취합매련반응（ PCR-RFLP）기술검측TNF-β G252A기인적기인빈솔，매련면역（ ELISA）법측정혈청TNF-β적함량，분석자궁내막이위증여TNFβ G252A기인지간적관계。결과：Ems조혈청TNF-β적함량명현고우대조조（ P<0.05），차Ems조혈청중TNF-β적함량여EmT적분기정정상관（ r=0.8532）；Ems조적A/A 기인형급G/A 기인형빈솔균명현고우대조조，차이유통계학의의（ P<0.05）；A/A기인형환Ems적위험도시G/G기인형적3.5배。결론：Ems환자혈청중TNF-β적함량여EmT적분기정정상관，A/A기인형시환Ems적위험기인형。
Objective:To investigate the role of TNF-βG252A gene in pathogenesis of endometriosis ( Ems ). Methods:Eighty Ems patients diagnosed by laparoscopy and histopathologic examination served as Ems group and 80 healthy women served as control group. PCR-RFLP was used to detect gene frequency of TNF-βG252A gene,and ELISA was applied to check the serum content of TNF-β. The relationship of Ems and TNF-β G252A gene was analyzed. Result:The serum level of TNF-β in Ems group was higher than that in control group( P<0 . 05 ),and the serum level of TNF-β in Ems group was positively associated with EmT period. A/A and G/A genotypic frequencies in Ems group were significantly higher than those in control group. The risk of Ems of A/A genotype was 3. 5 times of G/G genotype. Conclusions:The serum level of TNF-βin Ems patients is positively associated with EmT period,and A/A genotype is the risk genotype of Ems.
