中国老年保健医学
中國老年保健醫學
중국노년보건의학
CHINESE JOURNAL OF GERIATRIC CARE
2015年
2期
65-67
,共3页
吴冰%郑彦宏%侯振才%菅向东%崔勇
吳冰%鄭彥宏%侯振纔%菅嚮東%崔勇
오빙%정언굉%후진재%관향동%최용
乌司他丁%百草枯%急性肺损伤%保护作用
烏司他丁%百草枯%急性肺損傷%保護作用
오사타정%백초고%급성폐손상%보호작용
Ulinastatin%Paraquat%Acute lung injury%Protective effect
目的:通过建立百草枯中毒急性肺损伤大鼠模型,探讨乌司他丁( UTI)对百草枯( PQ)致急性肺损伤( ALI)的保护作用及可能机制。方法将健康Wister大鼠45只随机分为三组:对照组、PQ组、UTI组。对照组:腹腔注入生理盐水;PQ组:给予含百草枯(30mg/kg)的溶液经胃管注入;UTI组:百草枯染毒后30分钟腹腔注入含乌司他丁(100000u/kg)的等容量生理盐水。每组取8小时、24小时、48小时、72小时共4个观察点,测定血液及BALF中丙二醛( MDA)的含量、谷胱甘肽过氧化物酶( GSH-Px)和超氧化物歧化酶( SOD)活性。结果①PQ中毒组与对照组比较:中毒组血浆和BALF中MDA含量显著高于对照组,差异有显著性( P<0.01);血浆SOD活力GSH-Px均下降,差异有显著性( P<0.01);BALF中SOD、GSH-Px活力均下降,但48小时后SOD升高,差异有显著性(P<0.01)。②UTI治疗组与PQ中毒组比较:给予UTI治疗后的治疗组与中毒组比较,血浆中MDA含量明显下降(P<0.01),BALF中MDA含量下降(P<0.05);GSH-Px和SOD活力升高,差异有显著性( P<0.01)。结论 UTI对PQ中毒大鼠急性肺损伤具有保护作用,机制可能是减轻了肺组织的脂质过氧化反应。
目的:通過建立百草枯中毒急性肺損傷大鼠模型,探討烏司他丁( UTI)對百草枯( PQ)緻急性肺損傷( ALI)的保護作用及可能機製。方法將健康Wister大鼠45隻隨機分為三組:對照組、PQ組、UTI組。對照組:腹腔註入生理鹽水;PQ組:給予含百草枯(30mg/kg)的溶液經胃管註入;UTI組:百草枯染毒後30分鐘腹腔註入含烏司他丁(100000u/kg)的等容量生理鹽水。每組取8小時、24小時、48小時、72小時共4箇觀察點,測定血液及BALF中丙二醛( MDA)的含量、穀胱甘肽過氧化物酶( GSH-Px)和超氧化物歧化酶( SOD)活性。結果①PQ中毒組與對照組比較:中毒組血漿和BALF中MDA含量顯著高于對照組,差異有顯著性( P<0.01);血漿SOD活力GSH-Px均下降,差異有顯著性( P<0.01);BALF中SOD、GSH-Px活力均下降,但48小時後SOD升高,差異有顯著性(P<0.01)。②UTI治療組與PQ中毒組比較:給予UTI治療後的治療組與中毒組比較,血漿中MDA含量明顯下降(P<0.01),BALF中MDA含量下降(P<0.05);GSH-Px和SOD活力升高,差異有顯著性( P<0.01)。結論 UTI對PQ中毒大鼠急性肺損傷具有保護作用,機製可能是減輕瞭肺組織的脂質過氧化反應。
목적:통과건립백초고중독급성폐손상대서모형,탐토오사타정( UTI)대백초고( PQ)치급성폐손상( ALI)적보호작용급가능궤제。방법장건강Wister대서45지수궤분위삼조:대조조、PQ조、UTI조。대조조:복강주입생리염수;PQ조:급여함백초고(30mg/kg)적용액경위관주입;UTI조:백초고염독후30분종복강주입함오사타정(100000u/kg)적등용량생리염수。매조취8소시、24소시、48소시、72소시공4개관찰점,측정혈액급BALF중병이철( MDA)적함량、곡광감태과양화물매( GSH-Px)화초양화물기화매( SOD)활성。결과①PQ중독조여대조조비교:중독조혈장화BALF중MDA함량현저고우대조조,차이유현저성( P<0.01);혈장SOD활력GSH-Px균하강,차이유현저성( P<0.01);BALF중SOD、GSH-Px활력균하강,단48소시후SOD승고,차이유현저성(P<0.01)。②UTI치료조여PQ중독조비교:급여UTI치료후적치료조여중독조비교,혈장중MDA함량명현하강(P<0.01),BALF중MDA함량하강(P<0.05);GSH-Px화SOD활력승고,차이유현저성( P<0.01)。결론 UTI대PQ중독대서급성폐손상구유보호작용,궤제가능시감경료폐조직적지질과양화반응。
Objectives To establish rats model of acute lung injury by paraquat and study the protective effect of ulinastatin ( UTI) against paraquat( PQ)-induced acute lung injury( ALI) and its mechanism.Methods All 45 healthy adult Wister rats were randomizedly assigned into three groups:normal control group,PQ group,UTI group.①NS group( n=5):the same volume of 0.9%saline solution was orally administered to rats.②PQ group( n=20):20% paraquat(30mg/kg) was given by the oral cavity at the first day.③UTI group( n=20):ulinastatin (100 000u/kg) was injected into peritoneum at 30 minutes after oral administration of PQ.The content of maleic dialdehyde ( MDA) ,glutathione peroxidase( GSH-Px) and superoxide dismutase( SOD) activity in blood and bronchoalveolarlavage fluid(BALF)were routinely measured at the times of 8,24,48 and 72h respectively.Results ①Com-pared with control group,the level of MDA in PQ group increased significantly both in plasma and BALF(P<0.01);SOD and GSH-Px in plasma were all decreased (P<0.01).The activities of SOD and GSH-Px in BALF were all decreased,but SOD showed in-crease 48h later(P<0.01).②Compared UTI group with PQ group,the MDA level decreased both in plasma and BALF(P<0.05), the SOD and GSH-Px activities were significantly increased as well(P<0.01).Conclusions Ulinastatin alleviates paraquat(PQ)-induced acute lung injury in rats,which may be attributed to its attenuation of lipid peroxidation in the tissues.