CAJ | 학술논문

目的：了解贵州荔波少数民族人群雌激素受体（ ESRα）基因PvuⅡ位点和T29C位点多态性与乙型肝炎病毒（ HBV）易感性的关系。方法：选取贵州荔波县少数民族健康对照91例和HBsAg携带者80例，采用聚合酶链式反应-限制性片段长度多态性（ PCR-RFLP）方法测定受检者基因组中ESRα基因PvuⅡ和T29C位点多态性，分析两位点多态性与HBV易感性的关系。结果：在荔波少数民族健康对照组和HBsAg携带组中均检出ESRα基因PvuⅡ和T29C位点3种基因型，但3种基因频率和等位基因频率在两组间分布差异无统计学意义（P>0.05）。结论：ESRα基因的PvuⅡ和T29C基因多态性与荔波少数民族人群中乙肝感染无关。
목적：료해귀주려파소수민족인군자격소수체（ ESRα）기인PvuⅡ위점화T29C위점다태성여을형간염병독（ HBV）역감성적관계。방법：선취귀주려파현소수민족건강대조91례화HBsAg휴대자80례，채용취합매련식반응-한제성편단장도다태성（ PCR-RFLP）방법측정수검자기인조중ESRα기인PvuⅡ화T29C위점다태성，분석량위점다태성여HBV역감성적관계。결과：재려파소수민족건강대조조화HBsAg휴대조중균검출ESRα기인PvuⅡ화T29C위점3충기인형，단3충기인빈솔화등위기인빈솔재량조간분포차이무통계학의의（P>0.05）。결론：ESRα기인적PvuⅡ화T29C기인다태성여려파소수민족인군중을간감염무관。
Objective:Analysis on the correlation of the polymorphism of estrogen receptor( ESRα) gene PvuⅡ and T29 C loci with hepatitis B virus( HBV ) infection in Libo minority population of Guizhou province. Methods:Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP)technique was used to analyze the polymorphism of PvuⅡ and T29C loci in 91 healthy controls and 80 HBsAg carriers,the correlation of these two loci with susceptibility to HBV was ana-lyzed. Results:Three genotypes of ESRαPvuⅡand T29C loci were detected in both control group and HBsAg group. Between two groups,the three kinds of gene frequency and allele frequency distribution showed no statistical difference( P>0 . 05 ). Conclusions:The PvuⅡ and T29 C loci gene polymor-phism of ESR is not associated with HBV susceptibility in Libo minority nationality.