CAJ | 학술논문

目的：了解贵州省3个少数民族（布依族、水族、苗族）群体人类白细胞抗原-G（ HLA-G）基因14 bp插入/缺失多态性的分布规律及其与遗传背景之间的关系。方法：采用PCR扩增、电泳及测序的方法对来自贵州地区的布依族、水族、苗族共344例健康个体DNA进行HLA-G基因14 bp插入/缺失多态性检测，并与文献报道的仡佬族、壮族等13个民族群体的HLA-G基因14 bp插入/缺失多态性分布数据进行对比。结果：布依族、水族和苗族人群的+14 bp 等位基因频率分别为23.7%、26.0%和38.1%，+14 bp/+14 bp 基因型频率分别为9.40%、10.4%和15.9%，14 bp插入频率比较示布依族和苗族差异有统计学意义（χ2=11.345，P=0.001），苗族与水族差异有统计学意义（χ2=6.125，P=0.009）而布依族和水族间比较差异无统计学意义（ P>0.017）；与其他人群数据比较，苗族群体的14 bp插入/缺失分布与其他人群相似，而作为壮侗语族的布依族、水族则有自己独特的14 bp插入/缺失分布规律。结论：布依族、水族人群的14 bp插入/缺失分布相似，但是与苗族人群的分布存在一定的差异。
목적：료해귀주성3개소수민족（포의족、수족、묘족）군체인류백세포항원-G（ HLA-G）기인14 bp삽입/결실다태성적분포규률급기여유전배경지간적관계。방법：채용PCR확증、전영급측서적방법대래자귀주지구적포의족、수족、묘족공344례건강개체DNA진행HLA-G기인14 bp삽입/결실다태성검측，병여문헌보도적흘료족、장족등13개민족군체적HLA-G기인14 bp삽입/결실다태성분포수거진행대비。결과：포의족、수족화묘족인군적+14 bp 등위기인빈솔분별위23.7%、26.0%화38.1%，+14 bp/+14 bp 기인형빈솔분별위9.40%、10.4%화15.9%，14 bp삽입빈솔비교시포의족화묘족차이유통계학의의（χ2=11.345，P=0.001），묘족여수족차이유통계학의의（χ2=6.125，P=0.009）이포의족화수족간비교차이무통계학의의（ P>0.017）；여기타인군수거비교，묘족군체적14 bp삽입/결실분포여기타인군상사，이작위장동어족적포의족、수족칙유자기독특적14 bp삽입/결실분포규률。결론：포의족、수족인군적14 bp삽입/결실분포상사，단시여묘족인군적분포존재일정적차이。
Objective:To investigate the distribution of HLA-G gene 14 bp insertion /deletion poly-morphism in Buyi, Shui and Gelao populations and their relationship with genetic background. Methods:PCR amplification,agarose gel electrophoresis and sequencing were used to explore the dis-tribution of 14 bp insertion/deletion in HLA-G gene in 344 healthy people of these three ethnic groups, and the data were compared with that of 13 ethnic groups such as Gelao,Zuang reported in the litera-ture. Results:The frequencies of +14 bp in Buyi,Shui and miao were 23. 7%,26. 0% and 38. 1%respectively. The genotype frequencies of +14 bp/ +14 bp in Buyi,Shui and miao were 9. 40%, 10. 4% and 15. 9% respectively. The differences of 14 bp insertion frequencies between Buyi and mi-ao,between miao and Shui were statistically significant(P<0. 05),while no significant difference was found between Buyi and Shui( P >0. 05). When compared with other groups,the distribution characteristic of +14 bp insertion/deletion in miao was similar to them,but Buyi and Shui showed sig-nificance difference from others. Conclusion:The distribution characteristics of +14 bp insertion/de-letion in Buyi is similar to Shui,but they are different from that of miao.