贵阳医学院学报
貴暘醫學院學報
귀양의학원학보
JOURNAL OF GUIYANG MEDICAL COLLEGE
2015年
3期
229-233
,共5页
江涛%高婧%岳欢%高雅倩%黄俊琼
江濤%高婧%嶽歡%高雅倩%黃俊瓊
강도%고청%악환%고아천%황준경
白细胞介素31%基因敲除%聚合酶链反应( PCR)%反转录PCR%Western blot%模型,动物
白細胞介素31%基因敲除%聚閤酶鏈反應( PCR)%反轉錄PCR%Western blot%模型,動物
백세포개소31%기인고제%취합매련반응( PCR)%반전록PCR%Western blot%모형,동물
interleukin 31%gene knockout%polymerase chain reaction%reverse transcription-PCR%Western blot%model,animal
目的：建立IL-31RA基因敲除小鼠纯合子模型，为IL-31RA基因相关研究提供动物模型。方法：IL-31RA基因敲除小鼠严格按照SPF级要求的动物饲养标准进行饲养繁殖，采用聚合酶链式反应（ PCR）法鉴定子代小鼠的基因型，RT-PCR法鉴定小鼠IL-31RA mRNA的表达，Western blot鉴定IL-31RA蛋白的表达，HE染色观察小鼠重要脏器的形态学变化。结果：PCR法成功检测出子代小鼠的3种基因型，纯合子基因敲除小鼠未检测出IL-31RA mRNA和IL-31RA蛋白的表达，IL-31RA基因敲除小鼠的重要脏器的形态学特征与野生型小鼠比较无明显变化；基因敲除小鼠可成功饲养繁殖，亦可获得较多的基因敲除纯合子小鼠。结论：成功构建了IL-31RA基因敲除小鼠纯合子模型。
目的：建立IL-31RA基因敲除小鼠純閤子模型，為IL-31RA基因相關研究提供動物模型。方法：IL-31RA基因敲除小鼠嚴格按照SPF級要求的動物飼養標準進行飼養繁殖，採用聚閤酶鏈式反應（ PCR）法鑒定子代小鼠的基因型，RT-PCR法鑒定小鼠IL-31RA mRNA的錶達，Western blot鑒定IL-31RA蛋白的錶達，HE染色觀察小鼠重要髒器的形態學變化。結果：PCR法成功檢測齣子代小鼠的3種基因型，純閤子基因敲除小鼠未檢測齣IL-31RA mRNA和IL-31RA蛋白的錶達，IL-31RA基因敲除小鼠的重要髒器的形態學特徵與野生型小鼠比較無明顯變化；基因敲除小鼠可成功飼養繁殖，亦可穫得較多的基因敲除純閤子小鼠。結論：成功構建瞭IL-31RA基因敲除小鼠純閤子模型。
목적：건립IL-31RA기인고제소서순합자모형，위IL-31RA기인상관연구제공동물모형。방법：IL-31RA기인고제소서엄격안조SPF급요구적동물사양표준진행사양번식，채용취합매련식반응（ PCR）법감정자대소서적기인형，RT-PCR법감정소서IL-31RA mRNA적표체，Western blot감정IL-31RA단백적표체，HE염색관찰소서중요장기적형태학변화。결과：PCR법성공검측출자대소서적3충기인형，순합자기인고제소서미검측출IL-31RA mRNA화IL-31RA단백적표체，IL-31RA기인고제소서적중요장기적형태학특정여야생형소서비교무명현변화；기인고제소서가성공사양번식，역가획득교다적기인고제순합자소서。결론：성공구건료IL-31RA기인고제소서순합자모형。
Objective:To establish the IL-31RA gene knockout homozygous mice model and lay the foundation for further study on IL-31 gene. Methods:IL-31RA gene knockout mice were bred and re-produced according to the SPF class animal feeding standard. PCR was used to identify the genotype of the offspring,the expression of IL-31RA mRNA was detected by RT-PCR,expression of IL-31RA pro-tein was detected by Western blot,and morphological changes of vital organs were observed by HE staining . Result:Three genotypes of the offspring of IL-31 RA gene knockout mice were successfully i-dentified;expression of IL-31 RA mRNA and IL-31 RA protein was not detected in IL-31 RA gene knockout homozygous mice. Compared with the wild type mice,morphological characteristics of vital organs of had no significant changes in IL-31RA gene knockout homozygous mice. IL-31RA gene knockout mice could be dred and reproduced successfully. Conclusion:The IL-31RA gene knockout homozygous mice model has been successfully established.
