贵阳医学院学报
貴暘醫學院學報
귀양의학원학보
JOURNAL OF GUIYANG MEDICAL COLLEGE
2015年
3期
257-259,263
,共4页
弓形虫病,动物%感染%模型,动物%小鼠,近交ICR%妊娠
弓形蟲病,動物%感染%模型,動物%小鼠,近交ICR%妊娠
궁형충병,동물%감염%모형,동물%소서,근교ICR%임신
toxoplasmosis,animal%infection%models,animal%mice,inbred ICR%pregnancy
目的：探讨建立弓形虫生殖毒性妊娠期小鼠动物模型的方法。方法：将妊娠期ICR小鼠随机分为孕早期感染组、孕晚期感染组及相应对照组，孕早期和孕晚期感染组小鼠再分别分为3小组，分别以灌胃方式感染弓形虫PRU株包囊10、20及40个，对照组灌胃生理盐水，观察各组小鼠不良妊娠结局及产仔数量。结果：弓形虫PRU株感染数量为10个包囊时，孕早期感染组孕鼠的死亡率为36.36%，出现阴道流血、流产、早产等现象，不良妊娠结局高达85.71%，与对照组比较差异有统计学意义（ P<0.05）；感染20包囊和40包囊时，孕早期感染组孕鼠的死亡率高达50%和100%；孕晚期感染组孕鼠在妊娠期发病不明显，产仔鼠数量较对照组减少，但差异无统计学意义（ P>0.05）；结论：用10个PRU株包囊灌胃能建立妊娠期弓形虫感染小鼠动物模型，为研究弓形虫PRU株所致生殖毒性奠定基础。
目的：探討建立弓形蟲生殖毒性妊娠期小鼠動物模型的方法。方法：將妊娠期ICR小鼠隨機分為孕早期感染組、孕晚期感染組及相應對照組，孕早期和孕晚期感染組小鼠再分彆分為3小組，分彆以灌胃方式感染弓形蟲PRU株包囊10、20及40箇，對照組灌胃生理鹽水，觀察各組小鼠不良妊娠結跼及產仔數量。結果：弓形蟲PRU株感染數量為10箇包囊時，孕早期感染組孕鼠的死亡率為36.36%，齣現陰道流血、流產、早產等現象，不良妊娠結跼高達85.71%，與對照組比較差異有統計學意義（ P<0.05）；感染20包囊和40包囊時，孕早期感染組孕鼠的死亡率高達50%和100%；孕晚期感染組孕鼠在妊娠期髮病不明顯，產仔鼠數量較對照組減少，但差異無統計學意義（ P>0.05）；結論：用10箇PRU株包囊灌胃能建立妊娠期弓形蟲感染小鼠動物模型，為研究弓形蟲PRU株所緻生殖毒性奠定基礎。
목적：탐토건립궁형충생식독성임신기소서동물모형적방법。방법：장임신기ICR소서수궤분위잉조기감염조、잉만기감염조급상응대조조，잉조기화잉만기감염조소서재분별분위3소조，분별이관위방식감염궁형충PRU주포낭10、20급40개，대조조관위생리염수，관찰각조소서불량임신결국급산자수량。결과：궁형충PRU주감염수량위10개포낭시，잉조기감염조잉서적사망솔위36.36%，출현음도류혈、유산、조산등현상，불량임신결국고체85.71%，여대조조비교차이유통계학의의（ P<0.05）；감염20포낭화40포낭시，잉조기감염조잉서적사망솔고체50%화100%；잉만기감염조잉서재임신기발병불명현，산자서수량교대조조감소，단차이무통계학의의（ P>0.05）；결론：용10개PRU주포낭관위능건립임신기궁형충감염소서동물모형，위연구궁형충PRU주소치생식독성전정기출。
Objective:To establish a suitable pregnant mice infection model with Prugniaud( PRU) strain of Toxoplasma gondii. Methods:ICR mice were randomly divided into early-pregnancy infection group ,late-pregnancy infection group and the control group,and mices of each infection group were divided into 3 groups and were orally infected with 10,20 and 40 PRU strain cysts respectively,con-trol group was given normal saline instead. Adverse pregnancy outcomes and litter amount were ob-served. Results:When infected with 10 PRU strain cysts,mortality of early-pregnancy mice was 36. 6%,and vaginal bleeding,abortion,premature phenomena appeared,the incidence of adverse pregnancy outcomes reached to 85. 71%,there were significant differences compared with control group( P<0 . 05 ). mortality of early-pregnancy mice was 50% and 100% respectively when infected with 20 cyst and 40 cyst. The morbidity of mice in late-pregnancy infection group was low,and litter a-mount reduced,but compared with the control group,there was no significant difference(P>0. 05). Conclusions:Pregnant mice infection model is established with 10 PRU strain cysts of Toxoplasma gondii,which lays the foundation for the further study of reproductive toxicity of PRU strain.
