CAJ | 학술논문

目的：观察湿疹小鼠动物模型外周血中的 CD4+和 CD8+的水平，分析化湿汤结合润肤止痒膏对湿疹小鼠CD4+、CD8+及其比值的影响，探讨化湿汤结合润肤止痒膏治疗湿疹的机制。方法将60只健康成年昆明种小鼠随机分为空白对照组、湿疹模型组、湿疹阳性药物组、化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组，除空白对照组外，其余各组建立湿疹模型。空白对照组和湿疹模型组不进行治疗；湿疹阳性药物组用醋酸曲安奈德益康唑乳膏治疗，而化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组则采用相应药物治疗，每日1次。通过流式细胞仪对小鼠外周血中 T 淋巴细胞亚群进行测定。结果与湿疹模型组相比，湿疹阳性药物组、化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组小鼠外周血中 CD4+ T 淋巴细胞百分比及 CD4+ T/CD8+ T 均明显提高（P 均＜0.01），CD8+ T淋巴细胞百分比显著下降（P 均＜0.01）。与湿疹阳性药物组相比，化湿汤组、润肤止痒膏组小鼠外周血 CD4+ T 淋巴细胞百分比及CD4+ T/ CD8+ T 均明显降低（P 均＜0.01），CD8+T 淋巴细胞百分比显著升高（P 均＜0.01）；而化湿汤结合润肤止痒膏组小鼠外周血 CD4+ T 淋巴细胞、CD8+ T 淋巴细胞百分比及 CD4+T/ CD8+ T 无差异（P ＞0.05）。与化湿汤组、润肤止痒膏组相比，化湿汤结合润肤止痒膏组对小鼠外周血中 CD4+T 淋巴细胞百分比、CD8+T 淋巴细胞百分比及 CD4+T/ CD8+T的作用有显著差异（P＜0.01或＜0.05）。结论化湿汤结合润肤止痒膏可能是通过提高 CD4+T百分比，降低 CD8+ T淋巴细胞百分比以及提高 CD4+/CD8+，纠正了 CD4+T 淋巴细胞相对减少，CD8+T 淋巴细胞百分比相对增多，恢复了湿疹小鼠的细胞免疫功能和纠正了免疫失调。
목적：관찰습진소서동물모형외주혈중적 CD4+화 CD8+적수평，분석화습탕결합윤부지양고대습진소서CD4+、CD8+급기비치적영향，탐토화습탕결합윤부지양고치료습진적궤제。방법장60지건강성년곤명충소서수궤분위공백대조조、습진모형조、습진양성약물조、화습탕조、윤부지양고조급화습탕결합윤부지양고조，제공백대조조외，기여각조건립습진모형。공백대조조화습진모형조불진행치료；습진양성약물조용작산곡안내덕익강서유고치료，이화습탕조、윤부지양고조급화습탕결합윤부지양고조칙채용상응약물치료，매일1차。통과류식세포의대소서외주혈중 T 림파세포아군진행측정。결과여습진모형조상비，습진양성약물조、화습탕조、윤부지양고조급화습탕결합윤부지양고조소서외주혈중 CD4+ T 림파세포백분비급 CD4+ T/CD8+ T 균명현제고（P 균＜0.01），CD8+ T림파세포백분비현저하강（P 균＜0.01）。여습진양성약물조상비，화습탕조、윤부지양고조소서외주혈 CD4+ T 림파세포백분비급CD4+ T/ CD8+ T 균명현강저（P 균＜0.01），CD8+T 림파세포백분비현저승고（P 균＜0.01）；이화습탕결합윤부지양고조소서외주혈 CD4+ T 림파세포、CD8+ T 림파세포백분비급 CD4+T/ CD8+ T 무차이（P ＞0.05）。여화습탕조、윤부지양고조상비，화습탕결합윤부지양고조대소서외주혈중 CD4+T 림파세포백분비、CD8+T 림파세포백분비급 CD4+T/ CD8+T적작용유현저차이（P＜0.01혹＜0.05）。결론화습탕결합윤부지양고가능시통과제고 CD4+T백분비，강저 CD8+ T림파세포백분비이급제고 CD4+/CD8+，규정료 CD4+T 림파세포상대감소，CD8+T 림파세포백분비상대증다，회복료습진소서적세포면역공능화규정료면역실조。
Objective To observe the peripheral blood CD4+ and CD8+ of animal models in mice with eczema, analyze the influence of Huashi decoction combined with Runfuzhiyang cream for CD4+, CD8+ and its ratio in mice with eczema and explore the mechanism of Huashi decoction combined with Runfuzhiyang cream. Methods 60 healthy adult Kunming mice were randomly divided into blank control group, eczema model group, eczema positive drug group, Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group, eczema models were established in all groups except for the blank control group. Blank control group and eczema model group were not given treatment. Eczema positive drug group was treated by triamcinolone acetonide acetate econazole cream. Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group were treated by corresponding drug, 1 times daily. The T lymphocyte subpopulation in the mice peripheral blood was determined by flow cytometry. Results Compared with eczema model group, mice peripheral blood CD4+ T lymphocytes' percentage and CD4+/CD8+ T significantly increased (P all< 0.01), and CD8+ T lymphocytes' percentage decreased significantly (P all<0.01) in eczema positive drug group,Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group. Compared with eczema positive drug group, mice peripheral blood CD4+T lymphocytes' percentage and CD4+/CD8+ T were significantly lowered (P <0.01), CD8+ T lymphocytes' percentage increased significantly (P <0.01)in Huashi decoction group and Runfuzhiyang cream group; while mice peripheral blood CD4+ T lymphocytes, CD8+ T lymphocytes' percentage and CD4+T/CD8+ T had no difference(P>0.05) in Huashi decoction combined with Runfuzhiyang cream group. Compared with Huashi decoction group and Runfuzhiyang cream group, mice peripheral blood CD4+ T lymphocytes' percentage, CD8+ T lymphocytes' percentage and the effect of CD4+ T/CD8+ T had significant difference (P<0.01 or P <0.05) in the Huashi decoction combined with Runfuzhiyang cream group. Conclusion Huashi decoction combined with Runfuzhiyang cream may restore the cellular immune function and correct the immune disorders in mice with eczema by increasing the percentage of CD4+T, reducing the percentage of CD8+T lymphocytes and increasing CD4+/CD8+to correct the CD4+T lymphocytes decrease relatively and CD8+T lymphocytes' percentage increase relatively.