中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
10期
1516-1522
,共7页
陈立朋%晏平%郭天柱%侯婧瑛%郑韶欣%周长青%龙会宝%钟婷婷%王彤
陳立朋%晏平%郭天柱%侯婧瑛%鄭韶訢%週長青%龍會寶%鐘婷婷%王彤
진립붕%안평%곽천주%후청영%정소흔%주장청%룡회보%종정정%왕동
干细胞%移植%心肌干细胞%心律失常%心电生理学特性%室颤阈值%心肌梗死%国家自然科学基金
榦細胞%移植%心肌榦細胞%心律失常%心電生理學特性%室顫閾值%心肌梗死%國傢自然科學基金
간세포%이식%심기간세포%심률실상%심전생이학특성%실전역치%심기경사%국가자연과학기금
Myocytes,Cardiac%Heart Failure%Cardiac Electrophysiology%Ventricular Fibrilation
背景:课题组前期研究证实心肌干细胞治疗心肌梗死后短中期(6周)能明显改善心电生理学稳定性和室颤阈值。目的:观察心肌干细胞对心力衰竭大鼠室颤阈值和心电生理稳定性的长期影响。方法:开胸结扎20只SD大鼠左前降支冠状动脉,2周后其中10只大鼠局部梗死心肌内注射PKH26荧光标记的由PBS悬浮的心肌干细胞,另10只大鼠局部梗死心肌内注射等量PBS。治疗12周后,再次开胸检测梗死区、梗死边缘区和非梗死区的心电生理特性和室颤阈值。实验结束后,摘取心脏行病理切片,检查PHK26标记的心肌干细胞是否在梗死区内生存并表达连接蛋白43及α-肌动蛋白。结果与结论:与对照组相比,心肌干细胞移植12周后其梗死区、梗死边缘区和非梗死区的单极电图激动恢复时间、纠正的激动恢复时间差异无显著性意义(P >0.05);单极电图纠正的激动恢复时间离散度、电刺激所激发的恶性心律失常差异无显著性意义(P>0.05);室颤阈值方面,实验组梗死区和梗死边缘区与对照组相比差异无显著性意义(P >0.05),非梗死区与对照组相比差异有显著性意义(P <0.05)。PHK26标记的心肌干细胞在梗死边缘区内被发现并表达连接蛋白43和α-肌动蛋白。以上结果提示心肌干细胞移植后12周其心电生理的稳定性和室颤阈值的改善效应已逐渐消失,但机制未明。
揹景:課題組前期研究證實心肌榦細胞治療心肌梗死後短中期(6週)能明顯改善心電生理學穩定性和室顫閾值。目的:觀察心肌榦細胞對心力衰竭大鼠室顫閾值和心電生理穩定性的長期影響。方法:開胸結扎20隻SD大鼠左前降支冠狀動脈,2週後其中10隻大鼠跼部梗死心肌內註射PKH26熒光標記的由PBS懸浮的心肌榦細胞,另10隻大鼠跼部梗死心肌內註射等量PBS。治療12週後,再次開胸檢測梗死區、梗死邊緣區和非梗死區的心電生理特性和室顫閾值。實驗結束後,摘取心髒行病理切片,檢查PHK26標記的心肌榦細胞是否在梗死區內生存併錶達連接蛋白43及α-肌動蛋白。結果與結論:與對照組相比,心肌榦細胞移植12週後其梗死區、梗死邊緣區和非梗死區的單極電圖激動恢複時間、糾正的激動恢複時間差異無顯著性意義(P >0.05);單極電圖糾正的激動恢複時間離散度、電刺激所激髮的噁性心律失常差異無顯著性意義(P>0.05);室顫閾值方麵,實驗組梗死區和梗死邊緣區與對照組相比差異無顯著性意義(P >0.05),非梗死區與對照組相比差異有顯著性意義(P <0.05)。PHK26標記的心肌榦細胞在梗死邊緣區內被髮現併錶達連接蛋白43和α-肌動蛋白。以上結果提示心肌榦細胞移植後12週其心電生理的穩定性和室顫閾值的改善效應已逐漸消失,但機製未明。
배경:과제조전기연구증실심기간세포치료심기경사후단중기(6주)능명현개선심전생이학은정성화실전역치。목적:관찰심기간세포대심력쇠갈대서실전역치화심전생리은정성적장기영향。방법:개흉결찰20지SD대서좌전강지관상동맥,2주후기중10지대서국부경사심기내주사PKH26형광표기적유PBS현부적심기간세포,령10지대서국부경사심기내주사등량PBS。치료12주후,재차개흉검측경사구、경사변연구화비경사구적심전생리특성화실전역치。실험결속후,적취심장행병리절편,검사PHK26표기적심기간세포시부재경사구내생존병표체련접단백43급α-기동단백。결과여결론:여대조조상비,심기간세포이식12주후기경사구、경사변연구화비경사구적단겁전도격동회복시간、규정적격동회복시간차이무현저성의의(P >0.05);단겁전도규정적격동회복시간리산도、전자격소격발적악성심률실상차이무현저성의의(P>0.05);실전역치방면,실험조경사구화경사변연구여대조조상비차이무현저성의의(P >0.05),비경사구여대조조상비차이유현저성의의(P <0.05)。PHK26표기적심기간세포재경사변연구내피발현병표체련접단백43화α-기동단백。이상결과제시심기간세포이식후12주기심전생리적은정성화실전역치적개선효응이축점소실,단궤제미명。
BACKGROUND:Our previous work has demonstrated that the electrophysiological stability and ventricular fibrilation threshold were distinctly improved in rats with myocardial infarction 6 weeks after cardiac stem cels transplantation (short- to mid-term). OBJECTIVE: To observe the long-term effects of cardiac stem cels transplantation on the ventricular fibrilation threshold and electrophysiological stability in rats with heart failure post myocardial infarction.
<br> METHODS: Twenty Sprague-Dawley rats with myocardial infarction were induced by the left anterior descending coronary artery ligation. Two weeks later, animals were randomized to receive the injection of cardiac stem cels labeled with PKH26 in PBS or PBS alone into the local infarct zone. Twelve weeks after the cardiac stem cels or PBS injection, the electrophysiological characteristics and ventricular fibrilation threshold were measured at the infarct zone, the infarct marginal zone and the non-infarct zone. Labeled cardiac stem cels were detected and the expression of connexin-43 and α-sarcomeric actin was examined in cryostat sections from each harvested heart. RESULTS AND CONCLUSION:Compared with the PBS group, no significant differences were revealed in the unipolar electrogram activation recovery time and the correct activation recovery time in the infarct zone, the infarct marginal zone and the non-infarct zone in the cardiac stem cels group at 12 weeks post the cel transplantation (P > 0.05). There were no remarkable differences in the correct activation recovery time dispersion and induced malignant ventricular arrhythmias between the two groups (P > 0.05). No obvious differences were discovered in the ventricular fibrilation threshold in the infarct zone and infarct marginal zone in the cardiac stem cels group in contrast to the PBS group (P > 0.05), though a statistical significance could be found in the non-infarct zone between the two groups (P < 0.05). Labeled cardiac stem cels were identified in the infarct marginal zone and expressed connexin-43 and α-sarcomeric actin. It suggested that the beneficial effects of cardiac stem cels in modulating the electrophysiological stability and improving the ventricular fibrilation threshold faded away 12 weeks after cel transplantation. However, the mechanisms involved remained unclear.