CAJ | 학술논문

背景：研究显示骨质疏松多伴有成骨细胞的减少，成骨细胞替代疗法成为治疗骨质疏松症的新靶点。目的：观察人骨髓间充质干细胞在含地塞米松、维生素C及β-甘油磷酸钠培养基中向成骨细胞分化的能力。方法：采用人淋巴细胞分离液从成人骨髓血中分离纯化间充质干细胞，应用流式细胞仪检测其表面标志物的活性，透射电镜观察骨髓间充质干细胞的超微结构；将骨髓间充质干细胞在含有地塞米松、维生素 C 与β-甘油磷酸钠的成骨诱导培养基中诱导分化， RT-PCR检测骨髓间充质干细胞成骨诱导后骨形态发生蛋白2 mRNA的表达情况。结果与结论：培养2周后可见细胞呈成纤维状生长，强表达 CD44，CD29，不表达 CD34，CD45，具有向成骨细胞诱导分化的潜能，茜素红及碱性磷酸酶染色阳性，骨形态发生蛋白2 mRNA表达阳性，说明人骨髓间充质干细胞在含地塞米松、维生素C及β-甘油磷酸钠培养基中可向成骨细胞诱导分化，具有治疗骨质疏松症的潜能。
배경：연구현시골질소송다반유성골세포적감소，성골세포체대요법성위치료골질소송증적신파점。목적：관찰인골수간충질간세포재함지새미송、유생소C급β-감유린산납배양기중향성골세포분화적능력。방법：채용인림파세포분리액종성인골수혈중분리순화간충질간세포，응용류식세포의검측기표면표지물적활성，투사전경관찰골수간충질간세포적초미결구；장골수간충질간세포재함유지새미송、유생소 C 여β-감유린산납적성골유도배양기중유도분화， RT-PCR검측골수간충질간세포성골유도후골형태발생단백2 mRNA적표체정황。결과여결론：배양2주후가견세포정성섬유상생장，강표체 CD44，CD29，불표체 CD34，CD45，구유향성골세포유도분화적잠능，천소홍급감성린산매염색양성，골형태발생단백2 mRNA표체양성，설명인골수간충질간세포재함지새미송、유생소C급β-감유린산납배양기중가향성골세포유도분화，구유치료골질소송증적잠능。
BACKGROUND:Studies have shown that the number of osteoblasts is often decreased after osteoporosis, and osteoblast replacement therapy becomes a new target for the treatment of osteoporosis. OBJECTIVE:To observe the osteogenic differentiation of human bone marrow mesenchymal stem cels cultured in dexamethasone, vitamin C and beta-glycerophosphate. METHODS:Mesenchymal stem cels were isolated and purified from adult bone marrow using human lymphocyte separation medium. The expression of cel surface markers was detected by flow cytometry. Cel ultrastructure was observed by transmission electron microscope. Then, the bone marrow mesenchymal stem cels were cultured in osteogenic induction medium containing dexamethasone, vitamin C andβ-glycerophosphate, and RT-PCR was used to detect the bone morphogenetic protein-2 mRNA expression after osteogenic induction. RESULTS AND CONCLUSION:A large number of adherent cels were visible as fibrous growth at 2 weeks after culture and strongly expressed CD44, CD29, but did not express CD34, CD45. These cels could be induced to differentiate into osteoblasts, and express bone morphogenetic protein-2 mRNA. Alizarin red staining and alkaline phosphatase staining were positive for the cels. These findings suggest that human bone marrow mesenchymal stem cels cultured in dexamethasone, vitamin C and beta-glycerophosphate can differentiate into osteoblasts, and has a potential for the treatment of osteoporosis.