CAJ | 학술논문

目的探讨表面受体CD244在活动性肺结核患者CD8+T细胞中的功能.方法密度梯度离心法提取活动性肺结核患者和健康对照者的外周血单个核细胞,通过流式细胞术检测CD244在CD3+ CD8+细胞中的表达;通过细胞内染色方法检测CD244与细胞因子IFN-γ和TNF-α表达的关系.结果 CD244在活动性肺结核患者CD8+T细胞表达强度显著高于健康对照者(P=0.0003);复治肺结核患者的CD244表达强度显著高于新发肺结核患者(P=0.0011);CD244-细胞表达IFN-γ比例显著高于CD244+细胞(P=0.0013);CD244-细胞表达TNF-α比例显著高于CD244+细胞(P =0.0016).结论 CD244抑制活动性肺结核患者CD8+T细胞的细胞因子分泌表达.
목적 탐토표면수체CD244재활동성폐결핵환자CD8+T세포중적공능.방법 밀도제도리심법제취활동성폐결핵환자화건강대조자적외주혈단개핵세포,통과류식세포술검측CD244재CD3+ CD8+세포중적표체;통과세포내염색방법검측CD244여세포인자IFN-γ화TNF-α표체적관계.결과 CD244재활동성폐결핵환자CD8+T세포표체강도현저고우건강대조자(P=0.0003);복치폐결핵환자적CD244표체강도현저고우신발폐결핵환자(P=0.0011);CD244-세포표체IFN-γ비례현저고우CD244+세포(P=0.0013);CD244-세포표체TNF-α비례현저고우CD244+세포(P =0.0016).결론 CD244억제활동성폐결핵환자CD8+T세포적세포인자분비표체.
Objective To study the role of CD244 in CD8 + T cells of patients with active pulmonary tuberculosis.Methods Peripheral blood mononuclear cells (PBMCs) were isolated from patients with active tuberculosis and healthy controls.The expression of CD244 in CD8 + T cells was examined by flow cytometry.The relationships of CD244 with IFN-γ and TNF-α were analyzed by intracellular cytokine staining.Results The expression of CD244 in CD8 + T cells from patients with tuberculosis was significantly higher than that of healthy controls (P =0.0003),and the expression of CD244 in CD8 + T cells of retreatment tuberculosis patients was significantly higher than that of new patients(P =0.0011).The expression of IFN-γ and TNF-α was significantly higher in CD244-cells than that in CD244 + cells (P =0.0013,P =0.0016).Conclusions CD244 inhibited cytokine production of CD8 + T cells in patients with active pulmonary tuberculosis.