CAJ | 학술논문

背景：DCLK1是在有丝分裂后的神经元中发现的一种跨膜微管相关蛋白激酶，近些年来发现其可能是肠道肿瘤干细胞的标志物。目的：观察DCLK1与Ki67在结直肠肿瘤中的表达及其病理学意义，探讨是否可以将DCLK1+/Ki67-作为结直肠癌肿瘤干细胞的标志物。方法：运用免疫组织化学方法检测150例结直肠癌组织中DCLK1与Ki67的表达，并与正常肠黏膜组织、癌旁组织、腺瘤组织比较。结果与结论：DCLK1和Ki67在癌组织中的表达率分别为36.7%，34.7%，其数量明显高于正常肠黏膜和腺瘤组织；DCLK1的表达与部位、浸润深度、淋巴结转移有关(P <0.05)，而Ki67的表达仅与浸润深度有关(P <0.05)；DCLK1与 Ki67的表达呈负相关(r=-0.460，P=0.000)。结直肠癌组织中DCLK1+/Ki67-细胞数量约为2.01%，主要分布于肠黏膜隐窝的基底部及共壁腺管的共壁处，细胞核大深染且呈圆形或卵圆形，核仁明显，核分裂象少见，细胞质较少。DCLK1+/Ki67-细胞在不同分化程度的腺癌组织中均存在，且分化程度越低，细胞数量越多，故无论从细胞数量和位置，还是细胞形态，都表明DCLK1+/Ki67-细胞符合肿瘤干细胞的特征，可以将DCLK1+/Ki67-作为结直肠癌肿瘤干细胞的标志物。
배경：DCLK1시재유사분렬후적신경원중발현적일충과막미관상관단백격매，근사년래발현기가능시장도종류간세포적표지물。목적：관찰DCLK1여Ki67재결직장종류중적표체급기병이학의의，탐토시부가이장DCLK1+/Ki67-작위결직장암종류간세포적표지물。방법：운용면역조직화학방법검측150례결직장암조직중DCLK1여Ki67적표체，병여정상장점막조직、암방조직、선류조직비교。결과여결론：DCLK1화Ki67재암조직중적표체솔분별위36.7%，34.7%，기수량명현고우정상장점막화선류조직；DCLK1적표체여부위、침윤심도、림파결전이유관(P <0.05)，이Ki67적표체부여침윤심도유관(P <0.05)；DCLK1여 Ki67적표체정부상관(r=-0.460，P=0.000)。결직장암조직중DCLK1+/Ki67-세포수량약위2.01%，주요분포우장점막은와적기저부급공벽선관적공벽처，세포핵대심염차정원형혹란원형，핵인명현，핵분렬상소견，세포질교소。DCLK1+/Ki67-세포재불동분화정도적선암조직중균존재，차분화정도월저，세포수량월다，고무론종세포수량화위치，환시세포형태，도표명DCLK1+/Ki67-세포부합종류간세포적특정，가이장DCLK1+/Ki67-작위결직장암종류간세포적표지물。
BACKGROUND:DCLK1 is a transmembrane microtubule-associated kinase in neurons after mitotic division, which may be the intestinal cancer stem cel marker. OBJECTIVE:To observe the expression and pathological significance of DCLK1 and Ki67 in colorectal cancer. METHODS: Expression of Ki67 and DCLK1 in 150 cases of colorectal cancer tissues was detected by immunohistochemical method in contrast to normal colorectal mucosa, para-carcinoma tissue, and adenoma tissue. RESULTS AND CONCLUSION:The expression rates of DCLK1 and Ki67 were 36.7% and 34.7% in cancer tissues, respectively, both of which were significantly higher than those in normal colorectal mucosa and adenoma. The expression of DCLK1 was associated with the location, depth of invasion, lymph node metastasis (P < 0.05), while the expression of Ki67 was just associated with the depth of invasion (P < 0.05). There was a negative correlation between the expression of DCLK1 and Ki67 (r=-0.460,P=0.000). The count of DCLK1+/Ki67-cels was about 2.01% in colorectal cancer tissues, and these cels mainly distributed at the bottom of intestinal mucosa base and common duct wal. DCLK1+/Ki67- cels were oval, the nuclei were large and deep-stained with prominent nucleolus, and there was rare nuclear fission and less cytoplasm. From the aspects of cel number, location, and cel morphology, DCLK1+/Ki67- cels are in line with the characteristics of cancer stem cels; therefore, DCLK1+/Ki67-can be used as a cancer stem cel marker of colorectal cancer.