CAJ | 학술논문

目的：探讨肝门部胆管癌组织中骨形成蛋白3（BMP3）的表达情况及其临床意义。方法：收集30例肝门部胆管癌的临床标本，应用real-time PCR和Western blot检测BMP3 mRNA和蛋白在癌组织和癌旁组织的相对表达情况。收集103例肝门部胆管癌石蜡标本，免疫组织化学法检测肿瘤组织中BMP3蛋白的表达，并分析其与临床病理的关系。结果：全组30例肝门部胆管癌组织中，22例癌组织BMP3蛋白和mRNA的表达水平较癌旁组织明显下降。免疫组织化学方法显示103例肝门部胆管癌组织中，87例阴性，16例弱阳性，BMP3蛋白的表达与肿瘤TNM分期、淋巴结转移、肿瘤的分化程度密切相关（P＜0.05）。结论：肝门部胆管癌组织中BMP3基因处于抑制状态，其表达的下调可能与肝门部胆管癌的发生、发展密切相关。
목적：탐토간문부담관암조직중골형성단백3（BMP3）적표체정황급기림상의의。방법：수집30례간문부담관암적림상표본，응용real-time PCR화Western blot검측BMP3 mRNA화단백재암조직화암방조직적상대표체정황。수집103례간문부담관암석사표본，면역조직화학법검측종류조직중BMP3단백적표체，병분석기여림상병리적관계。결과：전조30례간문부담관암조직중，22례암조직BMP3단백화mRNA적표체수평교암방조직명현하강。면역조직화학방법현시103례간문부담관암조직중，87례음성，16례약양성，BMP3단백적표체여종류TNM분기、림파결전이、종류적분화정도밀절상관（P＜0.05）。결론：간문부담관암조직중BMP3기인처우억제상태，기표체적하조가능여간문부담관암적발생、발전밀절상관。
[ ABSTRACT ] AIM: To investigate the expression and clinical significance of bone morphogenetic protein 3 (BMP3) in hilar cholangiocarcinoma tissues.METHODS: Thirty cases of hilar cholangiocarcinoma specimens were col-lected.The expression of BMP3 at mRNA and protein levels in the tumor tissues and paracancerous tissues was detected by real-time PCR and Western blot.The hilar cholangiocarcinoma paraffin-embedded specimens (n=103) were collected. The protein expression of BMP3 was determined by immunohistochemical method, and the relationship of BMP3 protein ex-pression with clinical pathological characteristics was evaluated.RESULTS:In the 30 patients with hilar cholangiocarcino-ma, the expressions of BMP3 protein and mRNA in 22 cases of tumor tissues were significantly decreased compared with the adjacent normal tissues.The results of immunohistochemistry showed that 87 cases were negative and 16 cases were weakly positive in all 103 cases of hilar cholangiocarcinoma.The expression of BMP3 protein was associated with the tumor TNM staging, lymph node metastasis and tumor differentiation (P<0.05).CONCLUSION:BMP3 gene might be inhibited in human hilar cholangiocarcinoma.The down-regulation of BMP3 gene might be associated with the carcinogenesis and devel-opment of hilar cholangiocarcinoma.