中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2015年
4期
664-668
,共5页
任颖鸽%谭晓丽%陈静%张静%杜永平%张月萍
任穎鴿%譚曉麗%陳靜%張靜%杜永平%張月萍
임영합%담효려%진정%장정%두영평%장월평
芍药苷%缺氧%浦肯野细胞%膜电位%突触传递
芍藥苷%缺氧%浦肯野細胞%膜電位%突觸傳遞
작약감%결양%포긍야세포%막전위%돌촉전체
Paeoniflorin%Hypoxia%Purkinje cells%Membrane potential%Synaptic transmission
目的：研究芍药苷（paeoniflorin，Pae）对大鼠小脑浦肯野细胞（Purkinje cells，PCs）急性缺氧电生理反应的影响。方法：采用全细胞膜片钳记录法，记录大鼠小脑PCs膜电位、兴奋性和平行纤维（ parallel fibre，PF）-PC兴奋性突触后电流（excitatory postsynaptic currents，EPSCs），观察急性缺氧和芍药苷对上述电生理功能的影响。结果：缺氧后PCs首先表现为短暂的超极化，继之以短暂的去极化和持续超极化，芍药苷完全阻断了PCs的缺氧性超极化，并使PCs缺氧性去极化的幅度减小，持续时间缩短；缺氧上调了PCs兴奋性，芍药苷对缺氧引起的PCs的高兴奋性无显著影响；急性缺氧引起了PF-PC EPSCs的长时程抑制（ long-term depression，LTD）；芍药苷可部分逆转缺氧引起的PF-PC EPSCs衰减。结论：芍药苷显著减轻了大鼠小脑PCs的缺氧反应，可能增强了PCs对急性缺氧的耐受性。
目的：研究芍藥苷（paeoniflorin，Pae）對大鼠小腦浦肯野細胞（Purkinje cells，PCs）急性缺氧電生理反應的影響。方法：採用全細胞膜片鉗記錄法，記錄大鼠小腦PCs膜電位、興奮性和平行纖維（ parallel fibre，PF）-PC興奮性突觸後電流（excitatory postsynaptic currents，EPSCs），觀察急性缺氧和芍藥苷對上述電生理功能的影響。結果：缺氧後PCs首先錶現為短暫的超極化，繼之以短暫的去極化和持續超極化，芍藥苷完全阻斷瞭PCs的缺氧性超極化，併使PCs缺氧性去極化的幅度減小，持續時間縮短；缺氧上調瞭PCs興奮性，芍藥苷對缺氧引起的PCs的高興奮性無顯著影響；急性缺氧引起瞭PF-PC EPSCs的長時程抑製（ long-term depression，LTD）；芍藥苷可部分逆轉缺氧引起的PF-PC EPSCs衰減。結論：芍藥苷顯著減輕瞭大鼠小腦PCs的缺氧反應，可能增彊瞭PCs對急性缺氧的耐受性。
목적：연구작약감（paeoniflorin，Pae）대대서소뇌포긍야세포（Purkinje cells，PCs）급성결양전생리반응적영향。방법：채용전세포막편겸기록법，기록대서소뇌PCs막전위、흥강성화평행섬유（ parallel fibre，PF）-PC흥강성돌촉후전류（excitatory postsynaptic currents，EPSCs），관찰급성결양화작약감대상술전생리공능적영향。결과：결양후PCs수선표현위단잠적초겁화，계지이단잠적거겁화화지속초겁화，작약감완전조단료PCs적결양성초겁화，병사PCs결양성거겁화적폭도감소，지속시간축단；결양상조료PCs흥강성，작약감대결양인기적PCs적고흥강성무현저영향；급성결양인기료PF-PC EPSCs적장시정억제（ long-term depression，LTD）；작약감가부분역전결양인기적PF-PC EPSCs쇠감。결론：작약감현저감경료대서소뇌PCs적결양반응，가능증강료PCs대급성결양적내수성。
[ ABSTRACT] AIM:To investigate the effects of paeoniflorin ( Pae) on the functional responses induced by acute hypoxic insult in the rat cerebellar Purkinje cells ( PCs) .METHODS:The whole-cell patch clamp was used for the intra-cellular recording of PCs in the rat cerebellar slices to evaluate the changes of membrane potential, the excitability of PCs, and the parallel fibre ( PF)-PC excitatory postsynaptic currents ( EPSCs) upon acute hypoxic insult alone or with the pre-sence of Pae.RESULTS:PCs showed an initial hyperpolarization followed by brief depolarization and long lasting post-hy-poxia hyperpolarization after hypoxia exposure.Pae completely blocked hypoxia-induced hyperpolarization and decreased the amplitude and the duration of hypoxic depolarization.Hypoxia up-regulated the excitability of rat PCs.Pae didn’t show any significant effect on the hypoxia-induced hyperexcitability in PCs.Acute hypoxia induced long-term depression ( LTD) in rat cerebellar PF-PC EPSCs, and Pae partially reversed hypoxia-induced depression in PF-PC EPSCs.CONCLUSION:Pae significantly suppresses hypoxia-induced responses in rat PCs and probably increases the tolerance of rat PCs to acute hypoxia.
