CAJ | 학술논문

目的：探讨不同强度微波辐射对大鼠心脏的损伤作用，并初步探讨其作用机制。方法：分别以强度为500、1000、1500和2000 W／m2，频率为2450 MHz微波照射大鼠6 min，照射后6 h取大鼠心脏，检测心肌细胞中ATP含量、线粒体呼吸链复合体Ⅳ和Ⅴ活性，透射电镜观察心脏组织超微结构的改变，Muse细胞分析仪检测心肌细胞凋亡，Western blotting检测心肌细胞内cleaved caspase-3蛋白的表达。结果：随着微波辐射强度的增加，大鼠心肌细胞中ATP含量、线粒体呼吸链复合体Ⅳ和Ⅴ活性呈现降低趋势，与对照组相比，差异有统计学意义（ P＜0.05）。透射电镜结果显示，微波辐射致细胞线粒体数量减少且形态异常，大部分线粒体溶解空化，基质明显肿胀。心肌细胞凋亡检测结果显示，微波辐射能够诱导大鼠心肌细胞发生凋亡，且随着微波辐射强度的增加，细胞凋亡率呈上升趋势（P＜0.05）。 Western blotting结果显示，微波辐射后心肌细胞内cleaved caspase-3蛋白表达水平随着辐射强度的增加呈上升趋势（ P＜0.05）。结论：微波辐射对大鼠心脏具有明显的损伤作用，能够引起心脏能量代谢出现异常，诱发心肌细胞发生凋亡。
목적：탐토불동강도미파복사대대서심장적손상작용，병초보탐토기작용궤제。방법：분별이강도위500、1000、1500화2000 W／m2，빈솔위2450 MHz미파조사대서6 min，조사후6 h취대서심장，검측심기세포중ATP함량、선립체호흡련복합체Ⅳ화Ⅴ활성，투사전경관찰심장조직초미결구적개변，Muse세포분석의검측심기세포조망，Western blotting검측심기세포내cleaved caspase-3단백적표체。결과：수착미파복사강도적증가，대서심기세포중ATP함량、선립체호흡련복합체Ⅳ화Ⅴ활성정현강저추세，여대조조상비，차이유통계학의의（ P＜0.05）。투사전경결과현시，미파복사치세포선립체수량감소차형태이상，대부분선립체용해공화，기질명현종창。심기세포조망검측결과현시，미파복사능구유도대서심기세포발생조망，차수착미파복사강도적증가，세포조망솔정상승추세（P＜0.05）。 Western blotting결과현시，미파복사후심기세포내cleaved caspase-3단백표체수평수착복사강도적증가정상승추세（ P＜0.05）。결론：미파복사대대서심장구유명현적손상작용，능구인기심장능량대사출현이상，유발심기세포발생조망。
[ ABSTRACT] AIM:To investigate the effect of microwave radiation at different intensities on the rat myocardium and its possible mechanism.METHODS:The rats were radiated by the intensity of 500, 1 000, 1 500 and 2 000 W/m2 with 2 450 MHz microwave for 6 min.The heart tissue was collected 6 h after microwave radiation.ATP and mitochondria complexⅣandⅤwere measured.The changes of the tissue structures were observed under transmission electron micro-scope.The apoptosis of the myocardial cells was detected by a cell analyzer.The protein level of cleaved caspase-3 was de-termined by Western blotting.RESULTS:The concentration of ATP and activity of mitochondria complexⅣandⅤsigni-ficantly decreased compared with control group in the cardiac tissues.The decreased number, morphological abnormalities such as dissolved cavitation, matrix and obvious tumefaction of mitochondria were observed under transmission electron mi-croscope.The microwave radiation induced the apoptosis of myocardial cells in the rats.The cell apoptotic rate and the pro-tein level of cleaved caspase-3 increased with increasing intensity of microwave radiation ( P<0.05 ) .CONCLUSION:Microwave radiation has obvious injury effect on the rat heart, which can cause cardiac energy metabolism dysregulation and cardiac myocyte apoptosis.