CAJ | 학술논문

目的：通过研究太子参多糖（ Radix Pseudostellariae polysaccharide， RPP）减轻高脂肪诱导肝脏胰岛素抵抗的作用，探寻其主要影响因素及发生的分子机制。方法：随机将C57BL／6J小鼠分为低脂饲料对照组（ LFD组）和高脂饲料组（HFD组），16周后，经腹腔注射丙酮酸耐量试验（IPPTT）确定胰岛素抵抗糖代谢紊乱模型成功后，开始进行含RPP（500 mg／kg）的高脂饲料干预（HFD＋RPP组），连续干预4周之后，检测丙酮酸耐量以及肝脏组织和线粒体丙二醛（MDA）含量。同时采用Western blot法检测肝脏组织中p-AKT（Ser473／Thr308）、p-AMPK、核因子E2相关因子2（ Nrf2）、NQO1和IκBα的蛋白水平变化。结果：经RPP干预后，模型组小鼠血糖水平明显降低；肝脏和线粒体的MDA水平显著降低；肝脏组织p-AKT及p-AMPK的蛋白水平明显增高；NQO1、HO-1和IκBα蛋白水平也同时上调。结论：太子参多糖能够有效抑制高脂肪诱导的C57BL／6J小鼠高血糖作用，其机制可能与改善肝脏胰岛素信号转导，并减轻氧化应激反应，同时激活Nrf2信号通路及抑制肝脏炎症信号激活作用有关。
목적：통과연구태자삼다당（ Radix Pseudostellariae polysaccharide， RPP）감경고지방유도간장이도소저항적작용，탐심기주요영향인소급발생적분자궤제。방법：수궤장C57BL／6J소서분위저지사료대조조（ LFD조）화고지사료조（HFD조），16주후，경복강주사병동산내량시험（IPPTT）학정이도소저항당대사문란모형성공후，개시진행함RPP（500 mg／kg）적고지사료간예（HFD＋RPP조），련속간예4주지후，검측병동산내량이급간장조직화선립체병이철（MDA）함량。동시채용Western blot법검측간장조직중p-AKT（Ser473／Thr308）、p-AMPK、핵인자E2상관인자2（ Nrf2）、NQO1화IκBα적단백수평변화。결과：경RPP간예후，모형조소서혈당수평명현강저；간장화선립체적MDA수평현저강저；간장조직p-AKT급p-AMPK적단백수평명현증고；NQO1、HO-1화IκBα단백수평야동시상조。결론：태자삼다당능구유효억제고지방유도적C57BL／6J소서고혈당작용，기궤제가능여개선간장이도소신호전도，병감경양화응격반응，동시격활Nrf2신호통로급억제간장염증신호격활작용유관。
[ ABSTRACT] AIM:To study the role of Radix Pseudostellariae polysaccharide ( RPP) in hepatic insulin resist-ance.METHODS:Six-week-old C57BL/6J mice were randomly divided into low-fat diet (LFD) control group and high-fat diet ( HFD) model group.After 16 weeks, intraperitoneal pyruvate tolerance test ( IPPTT) was performed to determine the establishment of the HFD-induced hepatic insulin resistance model.HFD containing RPP (500 mg/kg) was given for 4 consecutive weeks.IPPTT, liver malondialdehyde ( MDA) level and liver mitochondrial MDA level were measured.The protein levels of p-AKT (Ser473/Thr308), p-AMPK, nuclear factor E2-related factor 2 (Nrf2), NQO1 and IκBαin the liver tissues were measured by Western blot.RESULTS:After administration of RPP, a significant reduction in the levels of blood glucose and hepatic mitochondrial MDA was observed.The levels of p-AKT ( Ser473/Thr308) and p-AMPK were significantly elevated in the liver tissues.The hepatic IκBαlevels were up-regulated.RPP also enhanced the expression of Nrf2 system-regulated proteins NQO1 and HO-1 in the liver tissues.CONCLUSION:Radix Pseudostellariae polysaccha-rides effectively reduce HFD-induced hepatic insulin resistance in C57BL/6J mice and improves liver glucose metabolism by ameliorating HFD-impaired hepatic transduction of insulin signaling, activating Nrf2-associated signaling and inhibiting the expression of inflammatory signaling proteins.