CAJ | 학술논문

背景：化疗药物可使处于生育年龄女性患者卵巢功能有不同程度的损伤，重则致卵巢早衰，已成为卵巢早衰发病的一个重要原因。因此，改善和恢复患者卵巢功能，已成为一个重要的课题。目的：探索骨髓间充质干细胞治疗化疗所致卵巢损伤的可行性及疗效。方法：建立化疗性卵巢衰竭模型，建模后注射PKH26标记后的骨髓间充质干细胞，于移植细胞后第15，30，45，60天，各取5只大鼠经尾静脉取血测卵泡刺激素、雌二醇水平并处死大鼠，留取右侧卵巢行常规病理切片，光学显微镜下记录卵巢卵泡数量变化。细胞移植后30 d，取2只大鼠与雄鼠合笼，观察生殖能力的差异。结果与结论：18%(4/22只)大鼠移植干细胞后动情周期能逐渐恢复，卵泡刺激素水平有所下降而雌二醇水平上升，卵巢病理切片提示卵泡数量减少，大鼠生育能力未受损害。结果表明骨髓间充质干细胞可以部分改善化疗后大鼠卵巢功能。
배경：화료약물가사처우생육년령녀성환자란소공능유불동정도적손상，중칙치란소조쇠，이성위란소조쇠발병적일개중요원인。인차，개선화회복환자란소공능，이성위일개중요적과제。목적：탐색골수간충질간세포치료화료소치란소손상적가행성급료효。방법：건립화료성란소쇠갈모형，건모후주사PKH26표기후적골수간충질간세포，우이식세포후제15，30，45，60천，각취5지대서경미정맥취혈측란포자격소、자이순수평병처사대서，류취우측란소행상규병리절편，광학현미경하기록란소란포수량변화。세포이식후30 d，취2지대서여웅서합롱，관찰생식능력적차이。결과여결론：18%(4/22지)대서이식간세포후동정주기능축점회복，란포자격소수평유소하강이자이순수평상승，란소병리절편제시란포수량감소，대서생육능력미수손해。결과표명골수간충질간세포가이부분개선화료후대서란소공능。
BACKGROUND:Chemotherapy drugs can damage the ovarian function in women of childbearing age, and even lead to premature ovarian failure. Therefore, to improve and restore the ovarian function in patients has become an important issue. OBJECTIVE:To explore the therapeutic effect and feasibility of bone marrow mesenchymal stem cel therapy against chemotherapy-induced ovarian damage. METHODS:Rat models of chemotherapy-induced premature ovarian failure were established, and injected with PKH26-labeled bone marrow mesenchymal stem cels. At 15, 30, 45, 60 days after cel transplantation, five rats were selected respectively to detect folicle-stimulating hormone and estradiol levels, and then, the rats were kiled to take the right ovary for pathological examination. The number of ovarian folicles was detected under light microscope. At 30 days after cel transplantation, another two rats were selected to mate with male rats to observe the difference in the reproductive activity. RESULTS AND CONCLUSION:Four of 22 rats (18%) gradualy recovered their estrous cycle after cel transplantation, with the decreased folicle-stimulating hormone level and increased estradiol level. Moreover, the number of folicles was reduced. Al of these indicated that the ability to have children in rats was not damaged.These experimental findings suggest that bone marrow mesenchymal stem cels can partialy improve the ovarian function of rats under chemotherapy.