中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
10期
1592-1596
,共5页
干细胞%移植%脐血间充质干细胞%低氧诱导因子1α%缺血再灌注损伤%脊髓
榦細胞%移植%臍血間充質榦細胞%低氧誘導因子1α%缺血再灌註損傷%脊髓
간세포%이식%제혈간충질간세포%저양유도인자1α%결혈재관주손상%척수
Cord Blood Stem Cel Transplantation%Hypoxia-Inducible Factor 1,alpha Subunit%Reperfusion Injury%Spinal Cord
背景：脊髓缺血再灌注损伤目前尚无有效治疗手段，多数研究集中于干细胞移植治疗。目的：观察肾下腹主动脉移植低氧诱导因子1α基因修饰的脐血间充质干细胞对脊髓缺血再灌注损伤大鼠的治疗作用。方法：30只成年SD雌性大鼠随机分为3组：对照组，未转染组，基因转染组，每组10只。手术阻断肾下腹主动脉1 h后恢复脊髓再灌注，分别经腹主动脉置管推注1 mL 10%PBS，1 mL脐血间充质干细胞悬液，1 mL低氧诱导因子1α修饰的脐血间充质干细胞悬液。术后1，6，12 d对大鼠进行BBB评分，Western blot法检测脊髓组织低氧诱导因子1α蛋白的表达，术后12 d行运动诱发电位测定。结果与结论：与对照组比较，未转染组和基因转染组大鼠BBB评分均显著升高(P <0.05)，脊髓组织低氧诱导因子1α蛋白表达明显上调(P <0.05)，运动诱发电位潜伏期缩短(P <0.05)，波幅增加(P <0.05)。与未转染组比较，基因转染组BBB评分明显升高(P <0.05)，脊髓组织低氧诱导因子1α蛋白表达上调(P <0.05)，运动诱发电位潜伏期缩短(P <0.05)，波幅增加(P <0.05)，结果表明低氧诱导因子1α修饰的脐血间充质干细胞治疗脊髓缺血再灌注损伤效果更加显著。
揹景：脊髓缺血再灌註損傷目前尚無有效治療手段，多數研究集中于榦細胞移植治療。目的：觀察腎下腹主動脈移植低氧誘導因子1α基因脩飾的臍血間充質榦細胞對脊髓缺血再灌註損傷大鼠的治療作用。方法：30隻成年SD雌性大鼠隨機分為3組：對照組，未轉染組，基因轉染組，每組10隻。手術阻斷腎下腹主動脈1 h後恢複脊髓再灌註，分彆經腹主動脈置管推註1 mL 10%PBS，1 mL臍血間充質榦細胞懸液，1 mL低氧誘導因子1α脩飾的臍血間充質榦細胞懸液。術後1，6，12 d對大鼠進行BBB評分，Western blot法檢測脊髓組織低氧誘導因子1α蛋白的錶達，術後12 d行運動誘髮電位測定。結果與結論：與對照組比較，未轉染組和基因轉染組大鼠BBB評分均顯著升高(P <0.05)，脊髓組織低氧誘導因子1α蛋白錶達明顯上調(P <0.05)，運動誘髮電位潛伏期縮短(P <0.05)，波幅增加(P <0.05)。與未轉染組比較，基因轉染組BBB評分明顯升高(P <0.05)，脊髓組織低氧誘導因子1α蛋白錶達上調(P <0.05)，運動誘髮電位潛伏期縮短(P <0.05)，波幅增加(P <0.05)，結果錶明低氧誘導因子1α脩飾的臍血間充質榦細胞治療脊髓缺血再灌註損傷效果更加顯著。
배경：척수결혈재관주손상목전상무유효치료수단，다수연구집중우간세포이식치료。목적：관찰신하복주동맥이식저양유도인자1α기인수식적제혈간충질간세포대척수결혈재관주손상대서적치료작용。방법：30지성년SD자성대서수궤분위3조：대조조，미전염조，기인전염조，매조10지。수술조단신하복주동맥1 h후회복척수재관주，분별경복주동맥치관추주1 mL 10%PBS，1 mL제혈간충질간세포현액，1 mL저양유도인자1α수식적제혈간충질간세포현액。술후1，6，12 d대대서진행BBB평분，Western blot법검측척수조직저양유도인자1α단백적표체，술후12 d행운동유발전위측정。결과여결론：여대조조비교，미전염조화기인전염조대서BBB평분균현저승고(P <0.05)，척수조직저양유도인자1α단백표체명현상조(P <0.05)，운동유발전위잠복기축단(P <0.05)，파폭증가(P <0.05)。여미전염조비교，기인전염조BBB평분명현승고(P <0.05)，척수조직저양유도인자1α단백표체상조(P <0.05)，운동유발전위잠복기축단(P <0.05)，파폭증가(P <0.05)，결과표명저양유도인자1α수식적제혈간충질간세포치료척수결혈재관주손상효과경가현저。
BACKGROUND:There are no available therapies for spinal cord ischemia-reperfusion injury, and stem cel transplantation is a focused topics. OBJECTIVE:To observe the therapeutic effect of hypoxia-inducible facotr-1α gene-modified umbilical cord blood mesenchymal stem cels (UC-MSCs) transplantedvia the infrarenal abdominal aorta on spinal cord ischemia-reperfusion injury in rats. METHODS:Thirty adult female Sprague-Dawley rats were randomly divided into three groups, with 10 rats in each group. The infrarenal abdominal aorta of rats was occluded surgicaly for 1 hour, and then the spinal cord reperfusion was restored. At 2 hours after reperfusion, 1 mL of 10% PBS, UC-MSCs suspension and hypoxia-inducible factor-1α-modified UC-MSCs suspension was injectedvia the infrarenal abdominal aorta, respectively, in the three groups. At 1, 6, 12 days after injection, Basso-Beattie-Bresnahan scores were recorded and western blot assay was used to detect hypoxia-inducible factor-1α protein expression in the spinal cord. The motor-evoked potential was determined at 12 days after injection. RESULTS AND CONCLUSION: Compared with the control group, the Basso-Beattie-Bresnahan scores were significantly higher (P < 0.05), the expression of hypoxia-inducible factor-1α protein in the spinal cord tissue was significantly increased (P < 0.05), the motor-evoked potential latency was shortened (P < 0.05) and the amplitude was increased (P< 0 .05) in the untransfected group and transfection group. Compared with the untransfected group, the Basso-Beattie-Bresnahan scores were significantly higher (P < 0.05), the expression of hypoxia-inducible factor-1α protein in the spinal cord tissue was significantly increased (P < 0.05), the motor-evoked potential latency was shortened (P < 0.05) and the amplitude was increased (P < 0 .05) in the transfection group. Above al, umbilical cord blood mesenchymal stem cel transplantation modified by hypoxia-inducible factor 1α has better effects on spinal cord ischemia-reperfusion injury.
