CAJ | 학술논문

背景：有多项研究证明，经静脉或经心肌表面注射移植间充质基质细胞促进了急性心肌梗死后心功能的改善。然而，目前尚缺乏有关经静脉联合经心肌注射移植间充质基质细胞对慢性心肌梗死心脏功能影响的研究。目的：探讨静脉联合心肌注射骨髓间充质基质细胞对慢性心肌梗死模型大鼠心脏功能的影响及其机制。方法：体外培养扩增Lewis大鼠的骨髓间充质基质细胞。将3×106 BrdU标记过的骨髓间充质基质细胞分别经股静脉和经心肌表面注射移植于慢性心肌梗死模型大鼠，对照组注射等量PBS。4周后检测两组大鼠心功能的变化，并对大鼠心脏组织行免疫组化染色，观察细胞分化情况及毛细血管数量。结果与结论：骨髓间充质基质细胞移植 4周后，细胞移植组与对照组心功能无明显差别，免疫组化染色提示存留于心肌组织内的移植细胞未分化为心肌细胞，心梗瘢痕区血管密度在细胞移植组和对照组间没有明显差别。结果表明经静脉联合经心肌注射骨髓间充质基质细胞未能改善慢性心肌梗死模型大鼠的心脏功能。
배경：유다항연구증명，경정맥혹경심기표면주사이식간충질기질세포촉진료급성심기경사후심공능적개선。연이，목전상결핍유관경정맥연합경심기주사이식간충질기질세포대만성심기경사심장공능영향적연구。목적：탐토정맥연합심기주사골수간충질기질세포대만성심기경사모형대서심장공능적영향급기궤제。방법：체외배양확증Lewis대서적골수간충질기질세포。장3×106 BrdU표기과적골수간충질기질세포분별경고정맥화경심기표면주사이식우만성심기경사모형대서，대조조주사등량PBS。4주후검측량조대서심공능적변화，병대대서심장조직행면역조화염색，관찰세포분화정황급모세혈관수량。결과여결론：골수간충질기질세포이식 4주후，세포이식조여대조조심공능무명현차별，면역조화염색제시존류우심기조직내적이식세포미분화위심기세포，심경반흔구혈관밀도재세포이식조화대조조간몰유명현차별。결과표명경정맥연합경심기주사골수간충질기질세포미능개선만성심기경사모형대서적심장공능。
BACKGROUND:It has been demonstrated to be effective for the improvement of heart function after acute myocardial infarction with intravenous or intramyocardial administration of bone marrow mesenchymal stromal cels. However, little is known regarding the effect of the combination of intravenous and intramyocardial administration of mesenchymal stromal cels on the heart function of a chronic myocardial infarction model. OBJECTIVE:To study the effect of intravenous and intramyocardial administration of bone marrow mesenchymal stromal cels on the heart function of a rat chronic myocardial infarction model and the relevant mechanism. METHODS:Bone marrow mesenchymal stromal cels isolated from Lewis rats were expandedex vivo. BrdU-labeled bone marrow mesenchymal stromal cels (3×106) were administeredvia the femoral vein and the myocardial surface respectively into rat models of chronic myocardial infarction in cel transplantation group. The equal volume of PBS was injected into the same place in control group. Four weeks after injection, echocardiography was performed to evaluate the heart function, and then the heart tissues were harvested for immunohistochemistry examination. The total blood vessel density in the scar area was evaluated. RESULTS AND CONCLUSION:At 4 weeks after cel implantation, the left ventricular function was not improved in the two groups. The immunohistochemistry staining showed that (1) the mesenchymal stromal cels in the myocardium did not differentiate to myocardial cels; (2) there was no significant difference in the total blood vessel density in the scar area between the cel transplantation and control groups. Taken together, the combined intravenous and intramyocardial administration of bone marrow mesenchymal stromal cels cannot improve heart function in a rat chronic myocardial infarction model.