CAJ | 학술논문

目的：检测蛋白酪氨酸磷酸酶非受体型22（PTPN22）基因选择性剪切亚型PTPN22．6 mRNA 在克罗恩病（CD）患者外周血单核细胞（PBMCs）中的表达水平，研究 PTPN22．6 mRNA 表达状况与 C 反应蛋白（CRP）和红细胞沉降率（ESR）的关系及其临床意义。方法采用实时定量聚合酶链反应（Real-time PCR）方法分析50例 CD 患者和35例对照组 PBMCs 中PTPN22．6 mRNA 表达。分别采用速率散射比浊法和全自动红细胞沉降系统分析仪测定 CRP 和ESR 水平。结果与对照组比较，CD 患者 PBMCs 中 PTPN22．6 mRNA 的表达量升高（P ＜0．05）。活动期 CD 患者 PBMCs 中 PTPN22．6 mRNA的表达量显著高于缓解期 CD 患者（P ＜0．05）。CD 患者 PBMCs 中 PTPN22．6 mRNA 表达量与 CRP 和 ESR 浓度呈正相关（r ＝0．356，P ＜0．01；r ＝0．512， P ＜0．01）。疾病行为与 CD 患者PBMCs中 PTPN22．6 mRNA 表达水平相关，梗阻型 CD 患者 PBMCs中 PTPN22．6 mRNA 表达量显著高于非狭窄非穿透型和穿透型 CD 患者（P ＜0．05）。结论CD 患者 PBMCs 中 PTPN22．6 mRNA 表达水平升高，高水平 PTPN22．6 mRNA 的表达不仅与梗阻型疾病行为和疾病活动状态相关，而且与 CRP 和 ESR 呈正相关，提示 PTPN22．6可能在 CD 免疫学发病中起重要作用。
목적：검측단백락안산린산매비수체형22（PTPN22）기인선택성전절아형PTPN22．6 mRNA 재극라은병（CD）환자외주혈단핵세포（PBMCs）중적표체수평，연구 PTPN22．6 mRNA 표체상황여 C 반응단백（CRP）화홍세포침강솔（ESR）적관계급기림상의의。방법채용실시정량취합매련반응（Real-time PCR）방법분석50례 CD 환자화35례대조조 PBMCs 중PTPN22．6 mRNA 표체。분별채용속솔산사비탁법화전자동홍세포침강계통분석의측정 CRP 화ESR 수평。결과여대조조비교，CD 환자 PBMCs 중 PTPN22．6 mRNA 적표체량승고（P ＜0．05）。활동기 CD 환자 PBMCs 중 PTPN22．6 mRNA적표체량현저고우완해기 CD 환자（P ＜0．05）。CD 환자 PBMCs 중 PTPN22．6 mRNA 표체량여 CRP 화 ESR 농도정정상관（r ＝0．356，P ＜0．01；r ＝0．512， P ＜0．01）。질병행위여 CD 환자PBMCs중 PTPN22．6 mRNA 표체수평상관，경조형 CD 환자 PBMCs중 PTPN22．6 mRNA 표체량현저고우비협착비천투형화천투형 CD 환자（P ＜0．05）。결론CD 환자 PBMCs 중 PTPN22．6 mRNA 표체수평승고，고수평 PTPN22．6 mRNA 적표체불부여경조형질병행위화질병활동상태상관，이차여 CRP 화 ESR 정정상관，제시 PTPN22．6가능재 CD 면역학발병중기중요작용。
Objective To explore the levels of protein tyrosine phosphatase nonreceptor type 22.6(PTPN22.6)mRNA in peripheral blood mononuclear cells(PBMCs)of Crohn’s disease(CD)pa-tients,and investigate its correlation with C-reactive protein (CRP)and erythrocyte sedimentation rate (ESR).Methods Levels of PTPN22.6 mRNA in PBMCs of 50 CD patients and 35 controls were detec-ted by real-time PCR,and levels of CRP and ESR were determined by rate nephelometry and automatic ESR Analyzer SRS 100 /II,respectively.Results Levels of PTPN22.6 mRNA expression were higher in CD patients than those in healthy controls(P <0.05).Of CD patients,compared with inactive cases,lev-els of PTPN22.6 mRNA expression increased in active cases(P <0.05);and levels of PTPN22.6 mRNA expression were significantly associated with the levels of CRP and ESR(r =0.356,P <0.01;r =0.512, P <0.01,respectively);compared with cases without stenosis,penetration or cases with penetration,cases with obstruction had significantly higher levels of PTPN22.6 mRNA(P =0.018;P <0.05,respectively). Conclusion PTPN22.6 mRNA expression levels increase in CD patients;high levels of PTPN22.6 mRNA are associated with the status of disease activity,CRP or ESR levels,and illness behavior.It sug-gests that PTPN22.6 could play a considerable role in pathogenesis of CD.