CAJ | 학술논문

目的：观察一氧化碳释放分子（CORM－2）对脓毒血症大鼠急性肾损伤的影响。方法：采用盲肠结扎穿孔术诱导大鼠脓毒症肾损伤模型，64只雄性SD 大鼠随机平均分为4组（n＝16），假手术组（Sham 组）、Sham 组＋CORM－2组，盲肠结扎穿孔（CLP）组及CLP＋CORM－2组。 CORM－2治疗组为盲肠结扎穿孔术后给予腹腔注射10mg／kg 剂量CORM－2。观察各组大鼠3d 累积生存率，术后12 h 测定肾脏组织中肌酐和尿素氮水平，同时检测IL－1β及TNF－α水平，观察各组大鼠24 h 时间点肾脏组织病理学变化。结果：与对照组（Sham 组，Sham＋CORM－2组）相比，CLP 组及 CLP＋CORM－2组大鼠生存率下降，肾组织肌酐、尿素氮升高，IL－1β及 TNF－α水平升高（P ＜0．05）。但与CLP 组相比，CLP＋CORM2可提高大鼠的3d 累积生存率，并改善脓毒症大鼠肾组织损伤，降低肾组织中IL－1β及TNF－α水平（P ＜0．05）。结论： CORM－2对脓毒症大鼠急性肾损伤有保护作用，其机制可能与CORM－2抑制脓毒症致肾损伤大鼠的炎性反应相关。
목적：관찰일양화탄석방분자（CORM－2）대농독혈증대서급성신손상적영향。방법：채용맹장결찰천공술유도대서농독증신손상모형，64지웅성SD 대서수궤평균분위4조（n＝16），가수술조（Sham 조）、Sham 조＋CORM－2조，맹장결찰천공（CLP）조급CLP＋CORM－2조。 CORM－2치료조위맹장결찰천공술후급여복강주사10mg／kg 제량CORM－2。관찰각조대서3d 루적생존솔，술후12 h 측정신장조직중기항화뇨소담수평，동시검측IL－1β급TNF－α수평，관찰각조대서24 h 시간점신장조직병이학변화。결과：여대조조（Sham 조，Sham＋CORM－2조）상비，CLP 조급 CLP＋CORM－2조대서생존솔하강，신조직기항、뇨소담승고，IL－1β급 TNF－α수평승고（P ＜0．05）。단여CLP 조상비，CLP＋CORM2가제고대서적3d 루적생존솔，병개선농독증대서신조직손상，강저신조직중IL－1β급TNF－α수평（P ＜0．05）。결론： CORM－2대농독증대서급성신손상유보호작용，기궤제가능여CORM－2억제농독증치신손상대서적염성반응상관。
Objective:To investigate the effects of carbon monoxide releasing molecules (CORM-2) on sepsis with acute kidney injury in rats. Methods: Sepsis kidney injury model was induced by cecal ligation and puncture 64 male SD rats. The rats were randomly divided into four groups (n = 16), sham-operated group (Sham group), Sham group + CORM-2 group, cecal ligation and puncture (CLP) group and CLP + CORM-2 group. CORM-2 treatment group were given intraperitoneal injection of 10 mg/kg dose of CORM-2 after cecal ligation and puncture. The three days cumulative survival rate was observed in four groups. The levels of blood urea nitrogen, creatinine, IL-1β and TNF-α of kidney tissue were detected after 12 h of CLP , and the renal histopathological changes were observed after 24 h of CLP in each group. Results: Compared with the control group (Sham group, Sham + CORM-2 group), CLP group and CLP + CORM-2 group were reduced survival rate and kidney creatinine , blood urea nitrogen , IL-1β and TNF-α significantly increased (P < 0.05). however, compared with CLP group, CLP + CORM-2 can improve the 3-day ssurvival rate of the rats and reduce septic rat kidney tissue injury , reduced renal tissue IL-1β and TNF-α levels (P <0.05). Conclusion:CORM-2 has a protective effect on septic acute kidney injury through inhibiting the inflammatory response in sepsis-induced renal injury in rats.