CAJ | 학술논문

为了探索从棉油皂脚中提取植物甾醇的工艺条件。以皂脚为原料，通过单因素和正交实验优化最佳工艺条件，并对粗品进行纯化，以高效液相色谱法测定的甾醇含量为考察指标。植物甾醇提取的最佳工艺为：皂化料液比1∶3，90℃下皂化3 h，用乙酸乙酯进行萃取，料液比为1∶20，粗甾醇的提取率为75.6%，其中提取得到的β-谷甾醇和菜油甾醇的纯度分别为（66.5±0.47）%、（5.67±0.08）%，其经无水乙醇重结晶两次后，纯度可达91.8%。结果表明最佳提取工艺重现性较好；HPLC法测定甾醇含量结果准确可靠。
위료탐색종면유조각중제취식물치순적공예조건。이조각위원료，통과단인소화정교실험우화최가공예조건，병대조품진행순화，이고효액상색보법측정적치순함량위고찰지표。식물치순제취적최가공예위：조화료액비1∶3，90℃하조화3 h，용을산을지진행췌취，료액비위1∶20，조치순적제취솔위75.6%，기중제취득도적β-곡치순화채유치순적순도분별위（66.5±0.47）%、（5.67±0.08）%，기경무수을순중결정량차후，순도가체91.8%。결과표명최가제취공예중현성교호；HPLC법측정치순함량결과준학가고。
The process of extracting phytosterols from Cottonseed Soapstock has been established. Methods:The conditions have been optimized through single factor and orthogonal experiments. The crude extract was purified and the content of phytosterols was determined by HPLC. Results:Optimum extraction conditions were as follows:the Liquid-material ratio is 1∶3;saponification time is 3 h at 90℃;the extraction was carried out with ethyl acetate and the liquid-material ratio is 1∶20;the yield of the crude phytosterols is 75.6%and the purity ofβ-sitosterol and campesterol is (66.5±0.47)%and (5.67±0.08)%, respectively. The purity of total phytosterols has been enhanced from 70 % to 91.8 % after recrystallization with anhydrous ethanol twice. Conclusion:The optimized extraction process indicates satisfied repeatability and the method for determining phytosterols by HPLC is accurate and reliable.