岭南急诊医学杂志
嶺南急診醫學雜誌
령남급진의학잡지
LINGNAN JOURNAL OF EMERGENCY MEDICINE
2015年
1期
1-2,7
,共3页
高糖%心肌成纤维细胞%碳酸酐酶2%凋亡
高糖%心肌成纖維細胞%碳痠酐酶2%凋亡
고당%심기성섬유세포%탄산항매2%조망
high glucose%cardiac fibroblasts%carbonic anhydrase 2%apoptosis%proliferation
目的：研究碳酸酐酶2（ CA2）在高糖刺激下的表达改变和对心肌成纤维细胞凋亡、增殖的影响。方法：SD 乳鼠心肌原代培养差速离心法分离心肌成纤维细胞，分为3组：正常糖（5．5 mmol／L，NG）组、高糖（25 mmol／L，HG）组、高糖加碳酸酐酶抑制剂乙氧苯唑胺（ETZ）组。细胞免疫荧光观察CA2的表达变化，MTS测定心肌成纤维细胞的增殖，Western blot检测bax、bcl－2、caspase3、carbonic anhydrase2的蛋白表达情况。结果：与 NG组相比，高糖能促进心肌成纤维细胞增殖和凋亡，并上调 CA2的表达，通过抑制 CA2可以抑制心肌成纤维细胞增殖和凋亡。结论：通过抑制CA2可减缓高糖诱导的心肌成纤维细胞增殖和凋亡。
目的：研究碳痠酐酶2（ CA2）在高糖刺激下的錶達改變和對心肌成纖維細胞凋亡、增殖的影響。方法：SD 乳鼠心肌原代培養差速離心法分離心肌成纖維細胞，分為3組：正常糖（5．5 mmol／L，NG）組、高糖（25 mmol／L，HG）組、高糖加碳痠酐酶抑製劑乙氧苯唑胺（ETZ）組。細胞免疫熒光觀察CA2的錶達變化，MTS測定心肌成纖維細胞的增殖，Western blot檢測bax、bcl－2、caspase3、carbonic anhydrase2的蛋白錶達情況。結果：與 NG組相比，高糖能促進心肌成纖維細胞增殖和凋亡，併上調 CA2的錶達，通過抑製 CA2可以抑製心肌成纖維細胞增殖和凋亡。結論：通過抑製CA2可減緩高糖誘導的心肌成纖維細胞增殖和凋亡。
목적：연구탄산항매2（ CA2）재고당자격하적표체개변화대심기성섬유세포조망、증식적영향。방법：SD 유서심기원대배양차속리심법분리심기성섬유세포，분위3조：정상당（5．5 mmol／L，NG）조、고당（25 mmol／L，HG）조、고당가탄산항매억제제을양분서알（ETZ）조。세포면역형광관찰CA2적표체변화，MTS측정심기성섬유세포적증식，Western blot검측bax、bcl－2、caspase3、carbonic anhydrase2적단백표체정황。결과：여 NG조상비，고당능촉진심기성섬유세포증식화조망，병상조 CA2적표체，통과억제 CA2가이억제심기성섬유세포증식화조망。결론：통과억제CA2가감완고당유도적심기성섬유세포증식화조망。
Objective: To investigate the role of carbonic anhydrase 2(CA2) on high glucose-induced apoptosis and proliferative rate in cardiac fibroblasts. Methods: Cardiac fibroblasts (CFs) cultured from Wistar rats were divided into groups as described as follow: normal glucose (5.5 mmol/L,NG)group, high glucose(25 mmol/L,HG) group and high glucose with CA2 inhibitor ethoxyzolamide (ETZ) treatment group. Immunofluorescence was used to detect the altered expression of CA2 in CFs. Western blot and MTS respectively used to explore expression of apoptosis-related proteins bax,bcl-2,caspase-3 and proliferative ability of CFs. Results: Compared to NG group,high glucose can up-regulate expression of CA2,bax,bcl-2,caspase-3. ETZ had the inhibitory effect on expressions of CA2 bax,bcl-2 and caspase-3 in high glucose-induced CFs. Conclusion: Inhibiting CA2 may alleviate apoptosis and improved proliferative ability of high glucose-induced cardiac fibroblasts.
