中南民族大学学报（自然科学版）
中南民族大學學報（自然科學版）
중남민족대학학보（자연과학판）
JOURNAL OF SOUTH-CENTRAL UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCE EDITION)
2015年
1期
33-35,42
,共4页
李晓华%杨振飞%李阳%陶新园%吴金龙%刘涛%陈鑫%梅枫%马婷婷
李曉華%楊振飛%李暘%陶新園%吳金龍%劉濤%陳鑫%梅楓%馬婷婷
리효화%양진비%리양%도신완%오금룡%류도%진흠%매풍%마정정
小单孢菌40027菌株%质粒pJTU101%抗性基因标记%稳定性
小單孢菌40027菌株%質粒pJTU101%抗性基因標記%穩定性
소단포균40027균주%질립pJTU101%항성기인표기%은정성
Micoromonospora sp.40027%plasmid pJTU101%resistance marker%genetic stability
从稀有放线菌小单孢菌40027菌株中分离到2个内源质粒pJTU101和pJTU112，用BamH I单酶切质粒pJTU101确定其大小，通过同源片段之间发生单交换实现内源质粒pJTU101的抗性标记，并对其稳定性进行了初步研究．结果表明：小单孢菌40027菌株内源质粒pJTU101拷贝数为1～2，大小约为36．6 kb，被阿泊拉霉素抗性基因标记且在小单孢菌40027菌株中比较稳定．
從稀有放線菌小單孢菌40027菌株中分離到2箇內源質粒pJTU101和pJTU112，用BamH I單酶切質粒pJTU101確定其大小，通過同源片段之間髮生單交換實現內源質粒pJTU101的抗性標記，併對其穩定性進行瞭初步研究．結果錶明：小單孢菌40027菌株內源質粒pJTU101拷貝數為1～2，大小約為36．6 kb，被阿泊拉黴素抗性基因標記且在小單孢菌40027菌株中比較穩定．
종희유방선균소단포균40027균주중분리도2개내원질립pJTU101화pJTU112，용BamH I단매절질립pJTU101학정기대소，통과동원편단지간발생단교환실현내원질립pJTU101적항성표기，병대기은정성진행료초보연구．결과표명：소단포균40027균주내원질립pJTU101고패수위1～2，대소약위36．6 kb，피아박랍매소항성기인표기차재소단포균40027균주중비교은정．
Two plasmids, pJTU101 and pJTU112, were isolated from Micoromonospora sp.40027.The size of plasmid pJTU101 was identified by BamH I restriction patterns and plasmid pJTU101 was labeled via single crossover between homologous fragments.The stability of pJTU101 was also studied.The results suggested that the plasmid pJTU101 with copy number of 1 ~2, which was about 36.6 kb, had high genetic stability with apramycin-resistant gene marker in Micoromonospora sp.40027.
