食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
6期
28-30,31
,共4页
酶解人参二醇%人参三醇%衍生化%高效液相色谱
酶解人參二醇%人參三醇%衍生化%高效液相色譜
매해인삼이순%인삼삼순%연생화%고효액상색보
enzymolysis%panoxadiol%panaxatriol%derivatization%HPLC
建立了高效液相法色谱测定人参和通过酶解工艺处理后的人参中的人参二醇和人参三醇的含量比较的方法。色谱条件：Diamonsil C18色谱柱，流动相为甲醇∶水，（95∶5）检测波长为230 nm。结果样品经提取，水解和衍生化等前处理后，人参二醇平均回收率为92.87%，RSD为2.18%（n=6）；人参三醇为平均回收率91.7%， RSD为2.10%（n=6）。人参二醇1.6 ug～16 ug内有良好的线性关系， r=0.9997。人参三醇1.6 ug～16 ug内有良好的线性关系， r=0.9991。
建立瞭高效液相法色譜測定人參和通過酶解工藝處理後的人參中的人參二醇和人參三醇的含量比較的方法。色譜條件：Diamonsil C18色譜柱，流動相為甲醇∶水，（95∶5）檢測波長為230 nm。結果樣品經提取，水解和衍生化等前處理後，人參二醇平均迴收率為92.87%，RSD為2.18%（n=6）；人參三醇為平均迴收率91.7%， RSD為2.10%（n=6）。人參二醇1.6 ug～16 ug內有良好的線性關繫， r=0.9997。人參三醇1.6 ug～16 ug內有良好的線性關繫， r=0.9991。
건립료고효액상법색보측정인삼화통과매해공예처리후적인삼중적인삼이순화인삼삼순적함량비교적방법。색보조건：Diamonsil C18색보주，류동상위갑순∶수，（95∶5）검측파장위230 nm。결과양품경제취，수해화연생화등전처리후，인삼이순평균회수솔위92.87%，RSD위2.18%（n=6）；인삼삼순위평균회수솔91.7%， RSD위2.10%（n=6）。인삼이순1.6 ug～16 ug내유량호적선성관계， r=0.9997。인삼삼순1.6 ug～16 ug내유량호적선성관계， r=0.9991。
To establisha high performance liquid chromatography (HPLC) method for simultaneous determination of residual panoxadiol and panaxatriol Ginseng and enzymolysis Ginseng by HPLC external standard method.HPLC gradient elution method was used withmethanol-water(95∶5).DiamonsiC18 column was used,and panoxadiol and panaxatriol was determined at the detection wavelength of 230 nm. The samples were treated by extraction , hydrolysis and derivation The averrage recovery and RSD of panoxadiol were 92.87%and 2.18%(n=6), respectively.The averrage recovery and RSD of panaxatriol were91.7%and 2.10%(n=6), respectively.The standard curve of panoxadiol was linear in the range of 1.6 ug-16 ug , r = 0.999 7and The standard curve of panaxatriol was linear in the range of 1.6 ug-16 ug , r=0.999 1.
