CAJ | 학술논문

为了建立酶解阿魏菇蛋白制备抗氧化多肽的最佳优化条件，采用胃蛋白酶和胰蛋白酶分步水解的方法。运用四因素三水平的正交试验确定胃蛋白酶的酶解优化组合，以水解度和DPPH自由基清除能力为响应值，最终确定酶解的最优组合为：酶解时间1 h，酶添加量8000 U/g，底物浓度1%，pH2。在此基础上，采用响应面分析法研究胰蛋白酶酶解时间、酶添加量、pH、底物浓度对工艺的影响，以羟自由基（·OH）为响应值，确定的酶解最优条件为：酶解时间1.42 h，酶添加量7744.93 U/g，pH7.96，底物浓度1.35%，此条件下的·OH清除率为74.7%，与理论值75.59%相对误差在1%以内，说明所建立的模型具有较好的拟合性。
위료건립매해아위고단백제비항양화다태적최가우화조건，채용위단백매화이단백매분보수해적방법。운용사인소삼수평적정교시험학정위단백매적매해우화조합，이수해도화DPPH자유기청제능력위향응치，최종학정매해적최우조합위：매해시간1 h，매첨가량8000 U/g，저물농도1%，pH2。재차기출상，채용향응면분석법연구이단백매매해시간、매첨가량、pH、저물농도대공예적영향，이간자유기（·OH）위향응치，학정적매해최우조건위：매해시간1.42 h，매첨가량7744.93 U/g，pH7.96，저물농도1.35%，차조건하적·OH청제솔위74.7%，여이론치75.59%상대오차재1%이내，설명소건립적모형구유교호적의합성。
In order to establish the optimum conditions of preparation antioxidant peptides from Pleurotus FerulaeLenzi protein, using pepsin and trypsin hydrolysis. The optimum technological conditions of pepsin hydrolysis were obtained by single factor and orthogonal array design methods , the degree of hydrolysis and DPPH radical scavenging capacity for response. The optimum conditions for pepsin hydrolysis method were as follows: hydrolysis time 1 h, enzyme dosage 8 000 U/g, substrate concentration 1 %, pH2. On this basis, utilizing response surface for analysis of tryptic hydrolysis time, enzyme dosage, pH, substrate concentration , hydroxyl radical (·OH) as a response to determine the optimal conditions for tryptic hydrolysis as follows:hydrolysis time 1.42 h, enzyme dosage 7 744.93 U/g, pH7.96, substrate concentration 1.35%, in this condition the ·OH clearance was 74.7 %, the relative error with the theoretical value 75.59 % was less than 1 %, indicating that that the model has a good fit of the experiment.