薤白多糖抑瘤及神经营养活性研究
해백다당억류급신경영양활성연구
The Antitumor Activity and Neurotrophic Activity of Polysaccharides from Allium macrostemon Bunge
  • 万方数据
    食品研究与开发 식품연구여개발
    FOOD RESEARCH AND CEVELOPMENT
    2015年 5期 107-110,115 ,共5页

    张占军%王富花%曾晓雄

    장점군%왕부화%증효웅

    薤白多糖%癌细胞%增殖%神经营养活性 薤白多糖%癌細胞%增殖%神經營養活性
    해백다당%암세포%증식%신경영양활성
    polysaccharide from Allium macrostemon bunge%cancer cells%proliferation%neurotrophic activity
    采用MTT法对薤白多糖纯化组分（AMP40-1、AMP40-2）在体外抑制人肺癌细胞A549和人胃癌细胞BGC-823的增殖作用进行了研究。结果显示，AMP40-2对人肺癌细胞A549的生长具有一定的抑制作用。对人胃癌细胞BGC-823的增殖实验结果表明，薤白多糖AMP40-1、AMP40-2均能不同程度的抑制BGC-823细胞的增殖，且抑制作用与多糖之间存在不同程度的正相关量效关系和时效关系。通过大鼠肾上腺嗜铬细胞瘤细胞PC12培养体系，对薤白多糖纯化组分（AMP40-1、AMP40-2）进行了神经营养活性研究。结果表明，薤白多糖AMP40-2对大鼠嗜铬细胞瘤PC12细胞具有一定的促分化作用。
    채용MTT법대해백다당순화조분（AMP40-1、AMP40-2）재체외억제인폐암세포A549화인위암세포BGC-823적증식작용진행료연구。결과현시，AMP40-2대인폐암세포A549적생장구유일정적억제작용。대인위암세포BGC-823적증식실험결과표명，해백다당AMP40-1、AMP40-2균능불동정도적억제BGC-823세포적증식，차억제작용여다당지간존재불동정도적정상관량효관계화시효관계。통과대서신상선기락세포류세포PC12배양체계，대해백다당순화조분（AMP40-1、AMP40-2）진행료신경영양활성연구。결과표명，해백다당AMP40-2대대서기락세포류PC12세포구유일정적촉분화작용。
    MTT assay was used to investigate the anti-proliferation activities against human gastric cancer cells (BGC-823) and human lung adenocarcinoma epithelial cells (A549) in vitro of AMP40-1 and AMP40-2. The results showed that AMP40-2 inhibited proliferation of A549 in a dose-dependent manner. For human gastric carcinoma cell BGC-823, AMP40-1 and AMP40-2 significantly inhibited proliferation in dose-dependent and time-dependent manner. The neurotrophic activity of polysaccharides from A. macrostemon Bunge were investingated by PC12 cell assay. The screening results showed that AMP40-2 induced the differentiation of PC12 cell and has obvious neurotrophic activity.
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