CAJ | 학술논문

运用实验法和数理统计法探究体育运动中结缔组织生长因子对肌腱干细胞分化产生的影响。将周围结缔组织从活体中进行细胞分离及培养；取P2代细胞爬片进行免疫细胞化学检测细胞表型抗原标记CD44、CD29。取P3代细胞进行肌腱干细胞多向分化能力鉴定，收集未处理的肌腱干细胞和分化后的肌腱细胞，分别用实时荧光定量PCR和Western印迹检测I型胶原和Tenascin-C mRNA和蛋白水平的表达。实验结果表明：细胞免疫荧光染色CD44、CD29阳性，表明我们分离提取的组织细胞为肌腱干细胞；肌腱干细胞可以向脂肪细胞、骨细胞、软骨细胞分化；分化后的肌腱细胞，I型胶原的mRNA水平含量升高了5.3倍，Tenascin-C升高了2.1倍(P<0.01)，I型胶原的蛋白表达升高了3.3倍，Tenascin-C的表达升高了1.8倍(P<0.05)。结论：结缔组织生长因子可以促进肌腱干细胞分化。
운용실험법화수리통계법탐구체육운동중결체조직생장인자대기건간세포분화산생적영향。장주위결체조직종활체중진행세포분리급배양；취P2대세포파편진행면역세포화학검측세포표형항원표기CD44、CD29。취P3대세포진행기건간세포다향분화능력감정，수집미처리적기건간세포화분화후적기건세포，분별용실시형광정량PCR화Western인적검측I형효원화Tenascin-C mRNA화단백수평적표체。실험결과표명：세포면역형광염색CD44、CD29양성，표명아문분리제취적조직세포위기건간세포；기건간세포가이향지방세포、골세포、연골세포분화；분화후적기건세포，I형효원적mRNA수평함량승고료5.3배，Tenascin-C승고료2.1배(P<0.01)，I형효원적단백표체승고료3.3배，Tenascin-C적표체승고료1.8배(P<0.05)。결론：결체조직생장인자가이촉진기건간세포분화。
To investigate the effect of Connective tissue growth factor(CTGF) on the differentiation of tendon stem/progenitor cells (TSPCs)in sports, this paper uses the experimental method and mathematical statistics method. Cells were isolated from the surrounding connective tissue of the living; the protein expressions of CD44、CD29 were detected by immunocy to chemistry staining from the cells at passage 2. Cells at passage 3 were used to demonstrate the multi-differentiation potential of the TSPCs, the mRNA expression of tenascin C and collagen type I were determined by real-time PCR, and the protein expressions of tenascin C and collagen type I were determined by Western blot. Results indicates that immunocy to chemistry staining showed that the protein expressions of CD44、CD29 were detected in cells we isolated; chondrogenic, osteogenic, and adi pogenic differentiation assays were used to demonstrate the multi-differentiation potential of the TSPCs; the mRNA expressions of collagen type I and Tenascin-C were 5.3 and 2.1 times higher than that in cells with no differentiation (P<0.01), the protein expressions of tenascin C and collagen type I were 3.3 and 1.8 times higher than that in cells with no differentiation (P<0.05).Conclusion: Connective tissue growth factor(CTGF)can be used to promote tenogenic differentiation of the TSPCs?in vitro.