食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
5期
29-34
,共6页
徐艳阳%蔡森森%张凡%李越山
徐豔暘%蔡森森%張凡%李越山
서염양%채삼삼%장범%리월산
桑白皮%植物甾醇%提取%树脂%柱色谱%薄层色谱
桑白皮%植物甾醇%提取%樹脂%柱色譜%薄層色譜
상백피%식물치순%제취%수지%주색보%박층색보
mulberry root bark%plant sterol%extraction%resin%column chromatography%thin layer chro-matography
为优化桑白皮甾醇的提取工艺，分别考查萃取溶剂种类、乙醇粗提液与蒸馏水体积比、萃取时间、乙醇粗提液与萃取溶剂体积比对桑白皮甾醇得率的影响；采用二因素三水平正交试验优化萃取工艺，结果表明最佳萃取工艺条件为：乙醇粗提液与氯仿体积比1∶1、乙醇粗提液与蒸馏水体积比1∶1，此时桑白皮甾醇得率为（5.63±0.28） mg/g。通过对不同类型大孔吸附树脂的比较，获得D-900大孔吸附树脂为较佳类型。氯仿萃取液采用D-900大孔吸附树脂进行柱层析两次，桑白皮甾醇纯度可达（50.48±1.39）%。对纯化后样品采用薄层色谱法进行定性分析，纯化样品和β-谷甾醇标准品在同一位置有紫红色斑点析出。应用气相色谱-质谱联用技术鉴定，桑白皮纯化样品含有羊毛甾醇、谷甾醇、β-香树脂醇和α-香树脂醇。
為優化桑白皮甾醇的提取工藝，分彆攷查萃取溶劑種類、乙醇粗提液與蒸餾水體積比、萃取時間、乙醇粗提液與萃取溶劑體積比對桑白皮甾醇得率的影響；採用二因素三水平正交試驗優化萃取工藝，結果錶明最佳萃取工藝條件為：乙醇粗提液與氯倣體積比1∶1、乙醇粗提液與蒸餾水體積比1∶1，此時桑白皮甾醇得率為（5.63±0.28） mg/g。通過對不同類型大孔吸附樹脂的比較，穫得D-900大孔吸附樹脂為較佳類型。氯倣萃取液採用D-900大孔吸附樹脂進行柱層析兩次，桑白皮甾醇純度可達（50.48±1.39）%。對純化後樣品採用薄層色譜法進行定性分析，純化樣品和β-穀甾醇標準品在同一位置有紫紅色斑點析齣。應用氣相色譜-質譜聯用技術鑒定，桑白皮純化樣品含有羊毛甾醇、穀甾醇、β-香樹脂醇和α-香樹脂醇。
위우화상백피치순적제취공예，분별고사췌취용제충류、을순조제액여증류수체적비、췌취시간、을순조제액여췌취용제체적비대상백피치순득솔적영향；채용이인소삼수평정교시험우화췌취공예，결과표명최가췌취공예조건위：을순조제액여록방체적비1∶1、을순조제액여증류수체적비1∶1，차시상백피치순득솔위（5.63±0.28） mg/g。통과대불동류형대공흡부수지적비교，획득D-900대공흡부수지위교가류형。록방췌취액채용D-900대공흡부수지진행주층석량차，상백피치순순도가체（50.48±1.39）%。대순화후양품채용박층색보법진행정성분석，순화양품화β-곡치순표준품재동일위치유자홍색반점석출。응용기상색보-질보련용기술감정，상백피순화양품함유양모치순、곡치순、β-향수지순화α-향수지순。
In order to optimize extraction process of plant sterol from mulberry root bark , effects of type of extraction solvent, volume ratio of ethanol crude extract to distilled water, extraction time, volume ratio of ethanol crude extract to extraction solvent on phytosterol extraction yield were investigated respectively. Then , optimization parameters were discussed by two-factor three-level orthoganol test analysis. Results showed that optimum extraction conditions were as follows:volume ratio of ethanol crude extract to chloroform of 1∶1, and ethanol crude extract to distilled water of 1∶1. Under these conditions, phytosterol extraction yield of (5.63± 0.28) mg/g was obtained. By comparison of different macroporous adsorption resin on purification effects , D-900 type of macroporus resin was choosed as purification column material. Extraction sample used by chloroform solution, reached a sterol purity of (50.48 ±1.39) %, purifed by twice of column chromatography. Purified extraction sample was qualititively analyzed by thin layer chromatography , Then purified extract and standard compound ofβ-sitosterol showed a similar maximum absorption peak at the same spots position on the thin layer chromatography plates. Lanosterol, sitosterol,β-amyrin andα-amyrin were identified in the purified exaction sample by Gas Chromatography-Mass spectrometry (GC-MS).
