CAJ | 학술논문

目的探讨脑出血小鼠学习能力、脑水肿改变及海马神经元电生理特性的变化规律. 方法选取80只雄性C57/BL6小鼠按随机数字表法分为脑出血组和对照组,其中脑出血组40只,对照组40只.脑出血组取20 μL尾动脉自体血,应用立体定位技术注入小鼠尾状核;对照组采用生理盐水同法注射.造模后1、3、5和7d对小鼠进行神经功能缺损评分评定,应用Morris水迷宫实验(定位航行实验、空间探索实验)观察小鼠的行为学表现,应用干湿重法检测脑组织含水量,应用全细胞膜片钳技术和计算机软件检测记录海马神经元电生理特性. 结果与对照组相比,造模后1、3、5和7d脑出血组小鼠神经功能缺损评分明显增高,训练第5天脑出血组小鼠在站台所在象限停留时间比率明显降低,造模后1、3、5和7d脑出血组小鼠血肿周围脑组织含水量明显升高;脑出血组小鼠海马CA1区锥体细胞的静息膜电位和输入电阻明显降低,差异均有统计学意义(P＜0.05). 结论小鼠发生脑出血后海马依赖的空间学习记忆能力降低,海马神经元钾离子通道的通透性增强.
목적 탐토뇌출혈소서학습능력、뇌수종개변급해마신경원전생리특성적변화규률. 방법 선취80지웅성C57/BL6소서안수궤수자표법분위뇌출혈조화대조조,기중뇌출혈조40지,대조조40지.뇌출혈조취20 μL미동맥자체혈,응용입체정위기술주입소서미상핵;대조조채용생리염수동법주사.조모후1、3、5화7d대소서진행신경공능결손평분평정,응용Morris수미궁실험(정위항행실험、공간탐색실험)관찰소서적행위학표현,응용간습중법검측뇌조직함수량,응용전세포막편겸기술화계산궤연건검측기록해마신경원전생리특성. 결과 여대조조상비,조모후1、3、5화7d뇌출혈조소서신경공능결손평분명현증고,훈련제5천뇌출혈조소서재참태소재상한정류시간비솔명현강저,조모후1、3、5화7d뇌출혈조소서혈종주위뇌조직함수량명현승고;뇌출혈조소서해마CA1구추체세포적정식막전위화수입전조명현강저,차이균유통계학의의(P＜0.05). 결론 소서발생뇌출혈후해마의뢰적공간학습기억능력강저,해마신경원갑리자통도적통투성증강.
Objective To establish mouse models of intracerebral hemorrhage using autologous arterial blood,to study the physiological property of hippocampal neurons,brain edema changes and learning ability in the mouse models after intracerebral hemorrhage.Methods Eighty male C57/BL6 mice were randomly divided into intracerebral hemorrhage group and control group (n=40); 20 μL arterial blood from the tail arteries or normal saline were injected into the caudate nucleus of intracerebral hemorrhage group and control group by stereotactic technique,respectively.One,three,five and seven d after injection,the neurological impairment was scored; the behavioral changes of the mice in the Morris water maze (navigation test and space exploration experiment) were observed; brain edema was measured by wet and dry weight method and electrophysiological differences of hippocampal neurons were recorded by whole-cell patch-clamp technique and computer software.Results As compared with those in the control group,significantly increased neurological deficit scores one,three,five and seven d after injection,statistically decreased residence time in the platform on the fifth d of training,obviously increased water content around the brain edema one,three,five and seven d after injection,and significantly decreased resting membrane potential and input resistance in the hippocampal CA1 pyramidal cells five d after injection of mice in the intracerebral hemorrhage group were noted (P＜0.05).Conclusion The hippocampus-dependent spatial leaming ability of intracerebral hemorrhage mice is decreased,and the permeability of potassium channels is enhanced.