CAJ | 학술논문

目的探讨高脂饮食饲养对小鼠前额皮层神经细胞线粒体DNA/mRNA稳定性的影响. 方法雄性小鼠40只按随机数字表法分为正常饮食组20只、高脂饮食组20只,各组饲养4个月、6个月后处死(各10只).应用常规试剂盒检测各时间点小鼠血清血糖、血脂及胆固醇水平变化;分离纯化神经细胞线粒体,利用普通PCR及实时定量PCR技术检测线粒体DNA/mRNA的变化. 结果 4个月时高脂饮食组小鼠血糖、血脂和胆固醇较正常饮食组差异无统计学意义(P＞0.05),6个月时高脂饮食组小鼠血脂水平较正常饮食组明显升高,差异有统计学意义(P＜0.05).4个月、6个月时高脂饮食组小鼠D-Loop水平与正常饮食组比较差异均无统计学意义(P＞0.05).与正常饮食组比较,6个月时高脂饮食组小鼠ATPase6水平降低,Cyt3和Cytb水平升高,差异有统计学意义(P＜0.05). 结论持续高脂饮食饲养6个月后小鼠神经细胞线粒体基因稳定性下降,首先是线粒体RNA表达模式异常.
목적 탐토고지음식사양대소서전액피층신경세포선립체DNA/mRNA은정성적영향. 방법 웅성소서40지안수궤수자표법분위정상음식조20지、고지음식조20지,각조사양4개월、6개월후처사(각10지).응용상규시제합검측각시간점소서혈청혈당、혈지급담고순수평변화;분리순화신경세포선립체,이용보통PCR급실시정량PCR기술검측선립체DNA/mRNA적변화. 결과 4개월시고지음식조소서혈당、혈지화담고순교정상음식조차이무통계학의의(P＞0.05),6개월시고지음식조소서혈지수평교정상음식조명현승고,차이유통계학의의(P＜0.05).4개월、6개월시고지음식조소서D-Loop수평여정상음식조비교차이균무통계학의의(P＞0.05).여정상음식조비교,6개월시고지음식조소서ATPase6수평강저,Cyt3화Cytb수평승고,차이유통계학의의(P＜0.05). 결론 지속고지음식사양6개월후소서신경세포선립체기인은정성하강,수선시선립체RNA표체모식이상.
Objective To investigate the effects of high fat diet on stability of mitochondrial DNA/mRNA of frontal cortex tissue in mice.Methods Forty male mice were randomly divided into regular diet group and high fat diet group (n=20).All animals were sacrificed at 4 months (n=10) or 6 months (n=10) after experiment.The serum glucose,lipid and cholesterol levels were evaluated.And mitochondria were isolated in the frontal cortex of mice,and the changes ofmitochondrial DNA/mRNA were tested using PCR and real-time quantitative PCR.Results The serum glucose,lipid and cholesterol levels showed no significant changes in the mice of high fat diet group after feeding for 4 months as compared with those in the mice of regular diet group (P＞0.05),while the lipid level in the high fat diet group was increased significantly as compared with that in the regular diet group after feeding for 6 months (P＜0.05).The D-Loop level showed no significant changes after feeding for 4 and 6 months between the high fat diet group and the regular diet group (P＞0.05).Decreased ATPase6 and increasd Cyt3 and Cytb expression levels in the high fat diet group were noted as compared with those in the regular diet group after feeding for 6 months(P＜0.05).Conclusion The stability of mitochondrial DNA decreases in the brain of mice at the persistent feeding with high fat diet for 6 months; especially,pattem of mitochondrial mRNA expression changes obviously at early stage.