CAJ | 학술논문

建立固相萃取-高效液相色谱法测定熏肠中苯并（α）芘的检测方法。样品经Cleanert BaP-3固相萃取柱净化、浓缩，C18色谱柱进行分离。结果表明：苯并（α）芘在线性范围0.5～10 ng/mL内，相关系数大于0.99，最低检出限为0.06μg/kg，相对标准偏差小于5%，回收率97.8%～107.0%。本方法快速简便、重现性好，可以用于熏肠中苯并（α）芘含量的测定。
건립고상췌취-고효액상색보법측정훈장중분병（α）비적검측방법。양품경Cleanert BaP-3고상췌취주정화、농축，C18색보주진행분리。결과표명：분병（α）비재선성범위0.5～10 ng/mL내，상관계수대우0.99，최저검출한위0.06μg/kg，상대표준편차소우5%，회수솔97.8%～107.0%。본방법쾌속간편、중현성호，가이용우훈장중분병（α）비함량적측정。
A high performance liquid chromatography with solid-phase extraction method was developed for the determination of benzo(α)pyrene in smoked sausage. The extracted sample was purified on a Cleanert BaP-3 solid-phase extraction column and separated on a C18 column. Benzo(α)pyrene was measured using a fluorescence detector at an excitation wavelength of 384 nm and an emission wavelength of 406 nm. The resultsshowed that benzo(α)pyrene displayed good linearity relationship over the concentration range of 0.5–10 ng/mL, with a coefficient correlation of 0.99. The limit of detection was 0.06μg/kg, and the precision was below 5%. The average recoveries for blank spiked samples were in the range of 97.8%—107.0%. This method was rapid, simple, reproducible, and applicable for benzo(α)pyrene determination in smoked sausage.