CAJ | 학술논문

建立了高效液相色谱紫外检测器测定大豆保健品中磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇的测定方法。采用NH2色谱柱（3.0 mm×150 mm×5μm），流动相为乙醇∶乙腈∶水=50∶40∶10（体积比），流速0.4 mL/min下，紫外检测器波长为206 nm处进行测定。样品经正己烷∶异丙醇=1∶1（体积比）混合液溶解并使用流动相稀释后直接进样分析。在线性范围内相关性良好，相关系数R2≥0.999，各添加水平回收率为91.12%～105.61%，RSD为4.85%，日内和日间精密度均小于4%，磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇定量限分别为1.5 mg/L、1.2 mg/L和0.9 mg/L。本方操作简单、准确、成本低，适合于分析大豆磷脂及其保健品中三种主要磷脂的含量。
건립료고효액상색보자외검측기측정대두보건품중린지선담감、린지선을순알、린지선기순적측정방법。채용NH2색보주（3.0 mm×150 mm×5μm），류동상위을순∶을정∶수=50∶40∶10（체적비），류속0.4 mL/min하，자외검측기파장위206 nm처진행측정。양품경정기완∶이병순=1∶1（체적비）혼합액용해병사용류동상희석후직접진양분석。재선성범위내상관성량호，상관계수R2≥0.999，각첨가수평회수솔위91.12%～105.61%，RSD위4.85%，일내화일간정밀도균소우4%，린지선담감、린지선을순알、린지선기순정량한분별위1.5 mg/L、1.2 mg/L화0.9 mg/L。본방조작간단、준학、성본저，괄합우분석대두린지급기보건품중삼충주요린지적함량。
A sensitive method for the determination of phosphatidylcholine , phosphatidyl ethanolamine and phosphatidylinositol in soybean phospholipids by high -performance liquid chromatographic analysis is described. A NH2 column (3.0 mm ×150 mm ×5 μm) was used and the isocratic elution mobile phase was ethanol∶acetonitrile∶water (50∶40∶10,v/v/v). The flow rate was 0.4 mL/min and the detector was UV with 206 nm. The sample was dissolved by n-hexane∶isopropanol(1∶1,v/v) and diluted by mobile phase for analyzed. The correlation coefficient of phosphatidylcholine , phosphatidyl ethanolamine and phosphatidylinositol was greater than 0.999 in linear range. The precision of interday and intraday of the three ingredients were less than 4 % and the recoveries were between 91.12 %-105.61 %. The limit of detection of phosphatidylcholine , phosphatidyl ethanolamine and phosphatidylinositol were 1.5 mg/L, 1.2 mg/L and 0.9 mg/L, respectively. The method is rapid, accurate and reliable, and can be used to determine three main components of soybean phospholipids and health care products.