食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
6期
86-88
,共3页
决明子茶%总黄酮%亚硝酸钠-氯化铝法%抗自由基活性%DPPH法
決明子茶%總黃酮%亞硝痠鈉-氯化鋁法%抗自由基活性%DPPH法
결명자다%총황동%아초산납-록화려법%항자유기활성%DPPH법
cassia seed tea%total flavonoid%NaNO2-AlCl3 method%anti-free radicals activity%DPPH method
以水、不同含量乙醇为溶剂，采用超声、回流等方法提取决明子茶中的总黄酮，采用亚硝酸钠-氯化铝法，通过紫外分光光度计在494 nm处测定样品吸收，计算总黄酮含量；采用DPPH法研究提取物抗自由基活性。研究发现超声和回流提取均含有一定量的黄酮，采用水回流提取的含量最低为3.39%，55%醇超声提取的含量最高为6.10%。决明子醇提取物对DPPH自由基有较好的清除作用，55%醇回流提取物自由基清除率为71.90%。
以水、不同含量乙醇為溶劑，採用超聲、迴流等方法提取決明子茶中的總黃酮，採用亞硝痠鈉-氯化鋁法，通過紫外分光光度計在494 nm處測定樣品吸收，計算總黃酮含量；採用DPPH法研究提取物抗自由基活性。研究髮現超聲和迴流提取均含有一定量的黃酮，採用水迴流提取的含量最低為3.39%，55%醇超聲提取的含量最高為6.10%。決明子醇提取物對DPPH自由基有較好的清除作用，55%醇迴流提取物自由基清除率為71.90%。
이수、불동함량을순위용제，채용초성、회류등방법제취결명자다중적총황동，채용아초산납-록화려법，통과자외분광광도계재494 nm처측정양품흡수，계산총황동함량；채용DPPH법연구제취물항자유기활성。연구발현초성화회류제취균함유일정량적황동，채용수회류제취적함량최저위3.39%，55%순초성제취적함량최고위6.10%。결명자순제취물대DPPH자유기유교호적청제작용，55%순회류제취물자유기청제솔위71.90%。
The total flavonoid of cassia seed tea were extracted with water or different ethanol water solution by ultrasonic or reflux. The total flavonoid contents were detected with absorption spectrophotometer in 494 nm by using NaNO2-AlCl3 method. The anti-free radical activity of the extracts was measured by using DPPH method. We found the flavonoid were detected in all of the extracts. The total flavonoid content in water extract by reflux was 3.39%, which was the lowest content.And that in 55%ethanol water solution extract by ultrasonic was 6.10%, which was the highest content. The extracts showed free radical scavenging activity to DPPH radical. And that in 55%ethanol water solution extract free radicals activity by reflux was 71.90%.
