CAJ | 학술논문

以藏区典型青稞品种藏青85为试验材料，以料液比、pH、提取温度和提取时间为单因素试验的基础上进行二次正交旋转组合设计，确定了适宜青稞蛋白提取的最佳工艺参数。结果表明，影响青稞蛋白质提取率的因素依次为pH、料液比、提取时间、提取温度，青稞蛋白质提取适宜工艺条件为：料液比1∶25（g/mL），pH 11，提取温度40℃，提取时间20 min，青稞蛋白质提取率为70.71%。
이장구전형청과품충장청85위시험재료，이료액비、pH、제취온도화제취시간위단인소시험적기출상진행이차정교선전조합설계，학정료괄의청과단백제취적최가공예삼수。결과표명，영향청과단백질제취솔적인소의차위pH、료액비、제취시간、제취온도，청과단백질제취괄의공예조건위：료액비1∶25（g/mL），pH 11，제취온도40℃，제취시간20 min，청과단백질제취솔위70.71%。
The quadratic orthogonal rotation combination design method was applied to optimize the extraction parameters of alkali extraction and acid precipitation method for isolation of highland barley (cv. Zangqing 85) protein. Influences of solid-to-liquid ratio, pH, extraction temperature and time on the protein extraction rate were studied respectively. The importance sequence of influence factors impacting protein extraction rate were pH, liquid ratio, extraction time and extraction temperature, successively. The optimum parameters of solid-to-liquid ratio , pH value , extraction temperature and time for protein extraction were:1∶25 , 11 , 40℃and 20 min, respectively. Under this optimal condition, the protein extraction rate reached to 70.71%.