CAJ | 학술논문

目的探讨二溴乙酸对小鼠免疫功能影响的机制.方法清洁级BALB/c小鼠40只,分为四组:即二溴乙酸5、20和50 mg/kg剂量组以及阴性对照.连续灌胃28 d后,检测脾和胸腺细胞因子白细胞介素(IL)-2、IL-4分泌水平,脾和胸腺内细胞凋亡率,以及凋亡相关基因(Fas,Bcl-2,TRAF-2和bax)和蛋白(Fas/FasL)的表达.结果与阴性对照组相比,二溴乙酸染毒组脾细胞的IL-2分泌水平明显增加(P＜0.01)而胸腺细胞的IL-2分泌水平降低(50 mg/kg剂量组有统计学意义,P＜0.05),脾细胞和胸腺细胞的IL-4的分泌水平明显降低(P＜0.01).脾和胸腺细胞凋亡率明显增加.胸腺和脾内的Fas和TRAF2基因表达显著增加,Fas/FasL的蛋白表达显著增加,具有统计学意义(P均为＜0.05).结论饮水中二溴乙酸对小鼠免疫器官的损伤是通过Fas/FasL途径诱导细胞凋亡.
목적 탐토이추을산대소서면역공능영향적궤제.방법 청길급BALB/c소서40지,분위사조:즉이추을산5、20화50 mg/kg제량조이급음성대조.련속관위28 d후,검측비화흉선세포인자백세포개소(IL)-2、IL-4분비수평,비화흉선내세포조망솔,이급조망상관기인(Fas,Bcl-2,TRAF-2화bax)화단백(Fas/FasL)적표체.결과 여음성대조조상비,이추을산염독조비세포적IL-2분비수평명현증가(P＜0.01)이흉선세포적IL-2분비수평강저(50 mg/kg제량조유통계학의의,P＜0.05),비세포화흉선세포적IL-4적분비수평명현강저(P＜0.01).비화흉선세포조망솔명현증가.흉선화비내적Fas화TRAF2기인표체현저증가,Fas/FasL적단백표체현저증가,구유통계학의의(P균위＜0.05).결론 음수중이추을산대소서면역기관적손상시통과Fas/FasL도경유도세포조망.
Objective To investigate the mechanism of immune function alteration induced by dibromoacetic acid(DBA) in mice.Methods:40 SPF(specific-pathogen-free) BALB/c mice were divided into four groups (control,DBA 5 mg/kg,20 mg/kg and 50 mg/kg).After intragastric administration for 28 days,the cytokine IL-2 and IL-4 secretion,the percentage of cell apoptosis and apoptosis related gene (Fas,Bcl-2,TRAF2 and bax) and protein (Fas/FasL) expression in the spleen and thymus were detected.Results DBA significantly increased the level of IL-2 in the spleen(P ＜ 0.01) but decreased in the thymus(P ＜ 0.05).The level of IL-4 secretion significantly decreased IL-2 in the spleen and thymus.The percentage of apoptosis increased in a dose-dependent manner after DBA exposure.The expression of Fas and TRAF2 genes increased significantly in thymus and spleen(P ＜ 0.05).The expression of Fas/FasL proteins were increased significantly in thymus and spleen(P ＜ 0.05).Conclusion DBA changes the cytokine level in the thymus and spleen.The immunecell apoptosis mediated by the Fas/FasL pathway may be the potential mechanism underlying this immunotoxicity.