CAJ | 학술논문

目的探讨应用脱氢野百合碱(DHMC)建立肺动脉高压犬模型的合适剂量.方法 32只比格犬按随机数字法分为4组:1.5 mg/kg DHMC组(n=8)、2.0 mg/kg DHMC组(n=8)、3.0 mg/kg DHMC组(n=8)和4.0 mg/kg DHMC组(n=8).各组实验犬行右颈静脉穿刺置入Swan-Ganz导管测量肺动脉收缩压、肺动脉平均压、肺血管阻力及心输出量,经右心室途径分别注射上述剂量的DHMC,处理8周后再次检测上述指标并进行肺组织病理学检查.结果处理8周后1.5 mg/kg DHMC组和2.0 mg/kg DHMC组实验犬全部存活,3.0 mg/kg DHMC组、4.0 mg/kg DHMC组实验犬死亡率分别为62.5%(5/8)和100.0%(8/8).与基础值比较,处理8周后1.5 mg/kg DHMC组实验犬肺动脉收缩压、肺动脉平均压、肺血管阻力及心输出量均无明显改变(均P>0.05).与同组基础值比较,处理8周后2.0 mg/kg DHMC组、3.0 mg/kg DHMC组存活实验犬肺动脉收缩压、肺动脉平均压及肺血管阻力均升高,心输出量降低(均P<0.05).结论 DHMC诱导肺动脉高压犬模型呈剂量相关性,小剂量(1.5 mg/kg)建模不成功,大剂量(4.0 mg/kg)死亡率高,推荐采用2.0~3.0 mg/kg剂量为宜.
목적 탐토응용탈경야백합감(DHMC)건립폐동맥고압견모형적합괄제량.방법 32지비격견안수궤수자법분위4조:1.5 mg/kg DHMC조(n=8)、2.0 mg/kg DHMC조(n=8)、3.0 mg/kg DHMC조(n=8)화4.0 mg/kg DHMC조(n=8).각조실험견행우경정맥천자치입Swan-Ganz도관측량폐동맥수축압、폐동맥평균압、폐혈관조력급심수출량,경우심실도경분별주사상술제량적DHMC,처리8주후재차검측상술지표병진행폐조직병이학검사.결과 처리8주후1.5 mg/kg DHMC조화2.0 mg/kg DHMC조실험견전부존활,3.0 mg/kg DHMC조、4.0 mg/kg DHMC조실험견사망솔분별위62.5%(5/8)화100.0%(8/8).여기출치비교,처리8주후1.5 mg/kg DHMC조실험견폐동맥수축압、폐동맥평균압、폐혈관조력급심수출량균무명현개변(균P>0.05).여동조기출치비교,처리8주후2.0 mg/kg DHMC조、3.0 mg/kg DHMC조존활실험견폐동맥수축압、폐동맥평균압급폐혈관조력균승고,심수출량강저(균P<0.05).결론 DHMC유도폐동맥고압견모형정제량상관성,소제량(1.5 mg/kg)건모불성공,대제량(4.0 mg/kg)사망솔고,추천채용2.0~3.0 mg/kg제량위의.
Objective To investigate the optimal dose of dehydromonocrotaline (DHMC) for establishing canine model of pulmonary hypertension.Methods Thirty-two beagle dogs were divided into 4 groups by random number:1.5 mg/kg DHMC group (n=8),2.0 mg/kg DHMC group (n=8),3.0 mg/kg DHMC group(n=8)and 4.0 mg/kg DHMC group(n=8).Each group underwent puncture with placement of Swan-Ganz catheter in the right jugular vein to measure the pulmonary arterial systolic pressure (PASP),mean pulmonary arterial pressure(MPAP),pulmonary vascular resistance(PVR)and cardiac output(CO).Then,each group of beagle dogs received injection of 1.5,2.0,3.0 and 4.0 mg/kg DHMC via right ventricle,respectively,depending on the dose assignment.All the indicators were measured again at 8 weeks after treatment,and the pathological examination of lung tissues was performed.Results At 8 weeks after treatment,all the experimental dogs in the 1.5 mg/kg DHMC group and 2.0 mg/kg DHMC group survived,whereas 62.5%(5/8)of the dogs in the 3.0 mg/kg DHMC group and 100.0%(8/8)of those in the 4.0 mg/kg DHMC group died.Compared with the baselines,the PASP,MPAP,PVR and CO in the experimental dogs of 1.5 mg/kg DHMC group did not show significant changes at 8 weeks after treatment(all P>0.05),while the PASP,MPAP,PVR were increased and the CO were decreased in the experimental dogs of 2.0 mg/kg DHMC group and 3.0 mg/kg DHMC group (all P<0.05).Conclusions DHMC-induced pulmonary hypertension in canine models is dose-related.The modeling may be unsuccessful with low-dose DHMC(1.5mg/kg)but highly-fatal with high-dose DHMC(4.0 mg/kg).An appropriate dose of 2.0-3.0 mg/kg DHMC is therefore recommended.