中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2015年
1期
40-41
,共2页
宋文卿%刘永锋%程颖%车彦鹏%梁世博
宋文卿%劉永鋒%程穎%車彥鵬%樑世博
송문경%류영봉%정영%차언붕%량세박
大鼠%胰岛%热缺血时间
大鼠%胰島%熱缺血時間
대서%이도%열결혈시간
Rat%Islet%Warm ischemia time
目的 观察不同热缺血时间对获取的大鼠胰岛及胰岛功能的影响.方法 采用雄性Wistar大鼠.在大鼠心跳停搏后,获取热缺血时间分别为0 min、15 min、30 min、45 min的4组大鼠胰腺,给予4℃的UW液保存8h,再将胶原酶溶液10 ml注入胰腺.胰岛获取后计算各组胰岛的纯度、活率及活性,并进行统计分析.结果 0 min组、15 min组、30 min组和45 min组获取的胰岛数分别为(433±41)、(396±38)、(350±31)和(66±17)IEQ/只,胰岛活率分别为94％、88％、77％和25％,胰岛纯度分别为88％、78％、60％和32％,胰岛素释放指数分别为2.38±0.23、2.25±0.18、2.19±0.18和1.25±0.12.与0 min组相比,15min组和30 min组获得的胰岛数量、纯度、活率及活性的差异均无统计学意义(P＞0.05),45 min组获得的胰岛数量、纯度、活率及活性与0 min组相比,差异有统计学意义(P＜0.05),低于临床上的胰岛移植标准(活性＞75％,纯度＞50％).结论 热缺血时间在30min以内对无心跳大鼠胰岛无明显影响,可用于胰岛移植.热缺血时间达45 min时,对大鼠胰岛可产生明显影响,不宜用于胰岛移植.
目的 觀察不同熱缺血時間對穫取的大鼠胰島及胰島功能的影響.方法 採用雄性Wistar大鼠.在大鼠心跳停搏後,穫取熱缺血時間分彆為0 min、15 min、30 min、45 min的4組大鼠胰腺,給予4℃的UW液保存8h,再將膠原酶溶液10 ml註入胰腺.胰島穫取後計算各組胰島的純度、活率及活性,併進行統計分析.結果 0 min組、15 min組、30 min組和45 min組穫取的胰島數分彆為(433±41)、(396±38)、(350±31)和(66±17)IEQ/隻,胰島活率分彆為94％、88％、77％和25％,胰島純度分彆為88％、78％、60％和32％,胰島素釋放指數分彆為2.38±0.23、2.25±0.18、2.19±0.18和1.25±0.12.與0 min組相比,15min組和30 min組穫得的胰島數量、純度、活率及活性的差異均無統計學意義(P＞0.05),45 min組穫得的胰島數量、純度、活率及活性與0 min組相比,差異有統計學意義(P＜0.05),低于臨床上的胰島移植標準(活性＞75％,純度＞50％).結論 熱缺血時間在30min以內對無心跳大鼠胰島無明顯影響,可用于胰島移植.熱缺血時間達45 min時,對大鼠胰島可產生明顯影響,不宜用于胰島移植.
목적 관찰불동열결혈시간대획취적대서이도급이도공능적영향.방법 채용웅성Wistar대서.재대서심도정박후,획취열결혈시간분별위0 min、15 min、30 min、45 min적4조대서이선,급여4℃적UW액보존8h,재장효원매용액10 ml주입이선.이도획취후계산각조이도적순도、활솔급활성,병진행통계분석.결과 0 min조、15 min조、30 min조화45 min조획취적이도수분별위(433±41)、(396±38)、(350±31)화(66±17)IEQ/지,이도활솔분별위94％、88％、77％화25％,이도순도분별위88％、78％、60％화32％,이도소석방지수분별위2.38±0.23、2.25±0.18、2.19±0.18화1.25±0.12.여0 min조상비,15min조화30 min조획득적이도수량、순도、활솔급활성적차이균무통계학의의(P＞0.05),45 min조획득적이도수량、순도、활솔급활성여0 min조상비,차이유통계학의의(P＜0.05),저우림상상적이도이식표준(활성＞75％,순도＞50％).결론 열결혈시간재30min이내대무심도대서이도무명현영향,가용우이도이식.열결혈시간체45 min시,대대서이도가산생명현영향,불의용우이도이식.
Objective To observe the influence of warm ischemia time on acquisition of rat pancreatic islets and islet function.Method Male Wistar rats were used.After heart beats stopped,the pancreases in four groups of rats were harvested,and warm ischemia time was 0,15,30 and 45 min separately.The pancrease was preserved in UW at 4℃C for 8 h,and subjected to injection of collagenase solutions.After islets were acquired,the purity,survival rate and islet activity were tested,and statistical analysis was performed.Result The number of islets obtained in 0 min group,15 min group,30 min group and 45 min group was (433 ± 41),(396 ± 38),(350 ± 31) and (66 ± 17)IEQ/one,islet viability was 94％,88％,77％ and 25％,and purity was 88％,78％,60％ and 32％,and insulin release index was 2.38 ± 0.23,2.25 ± 0.18,2.19-± 0.18 and 1.25 ± 0.12,respectively.There was no significant difference in islet number,purity,survival rate and activity 15 min group and 30 min group between 15 min group or 30 min group and 0 min group (P＞0.05).There was significant difference between 45 min group and 0 min group in islet number,purity,survival rate and activity (P＜0.05).The survival rate and purity in 45 min group were lower than the clinical standards for islet transplantation (survival rate ＞ 75％,and purity ＞ 50％).Conclusion Warm ischemia time of 15 min in non-heart-beating brain death(NHBD) rats had no effect on islet isolation and purification.Warm ischemia time within 30 min showed no significant influence on islets of NHBD rats,which can be used in islet transplantation.Warm ischemia time at 45 min showed significant influence on islets of NHBD rats,which can't be used in islet transplantation.
