CAJ | 학술논문

目的探讨细胞毒性T淋巴细胞相关抗原4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)基因多态性与青海省汉族人群Graves眼病(Graves ophthalmopathy,GO)的相关性.方法选择2011年6月至2014年2月在青海省人民医院糖尿病科确诊的汉族Graves病患者90例作为观察对象,并将其分为GO组(49例)与无眼病(GD)组(41例).采用限制性片段长度多态性聚合酶链反应(PCR-RFLP),检测观察对象血清中CTLA-4基因外显子1的49位点A/G基因型及等位基因.结果 GO、GD组人群CTLA-4基因外显子1的49位点A/G基因型(AA、AG、GG)频率比较,差异无统计学意义[4.1％(2/49)比7.3％(3/41),44.9％(22/49)比61.0％(25/41),51.0％ (25/49)比31.7％(13/41),Fisher确切概率法,P=0.180＞ 0.05];等位基因(A、G)频率比较,差异无统计学意义[26.5％(26/98)比37.8％(31/82),73.5％(72/98)比62.2％(51/82),x2=2.622,P＞0.05].结论 CTLA-4基因多态性与青海省汉族人群GO的发生无相关性.
목적 탐토세포독성T림파세포상관항원4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)기인다태성여청해성한족인군Graves안병(Graves ophthalmopathy,GO)적상관성.방법 선택2011년6월지2014년2월재청해성인민의원당뇨병과학진적한족Graves병환자90례작위관찰대상,병장기분위GO조(49례)여무안병(GD)조(41례).채용한제성편단장도다태성취합매련반응(PCR-RFLP),검측관찰대상혈청중CTLA-4기인외현자1적49위점A/G기인형급등위기인.결과 GO、GD조인군CTLA-4기인외현자1적49위점A/G기인형(AA、AG、GG)빈솔비교,차이무통계학의의[4.1％(2/49)비7.3％(3/41),44.9％(22/49)비61.0％(25/41),51.0％ (25/49)비31.7％(13/41),Fisher학절개솔법,P=0.180＞ 0.05];등위기인(A、G)빈솔비교,차이무통계학의의[26.5％(26/98)비37.8％(31/82),73.5％(72/98)비62.2％(51/82),x2=2.622,P＞0.05].결론 CTLA-4기인다태성여청해성한족인군GO적발생무상관성.
Objective To investigate the association between gene polymorphism of cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) and Graves ophthalmopathy (GO) in Qinghai Han population.Methods Ninety cases of Graves disease were selected from June 2011 to February 2014 in The People's Hospital of Qinghai Province,and the 90 patients were divided into two subgroups according to GO (49 cases) and GD without GO(41 cases).Then the genotype and allele of CTLA-4 exon 1 (+ 49A/G) were detected in surum by the method of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).Results The distribution of CTLA-4 exon 1 (+ 49 A/G) genotype frequencies (AA,AG,GG) was not different between GO and GD without GO subgroups [4.1％ (2/49) to 7.3％ (3/41),44.9％ (22/49) to 61.0％ (25/41),51.0％ (25/49) to 31.7％ (13/41),Fisher exact probability,P =0.180 ＞ 0.05]; the distribution of CTLA-4 exon 1 (+ 49A/G) allele frequencies (A,G) was not different between GO and GD without GO subgroups [26.5％ (26/98) to 37.8％ (31/82),73.5％ (72/98) to 62.2％ (51/ 82),x2 =2.622,P＞ 0.05].Conclusion CTLA-4 gene exon 1 (+ 49A/G) may not be a candidate susceptibility gene for Qinghai Han GO.