CAJ | 학술논문

目的探讨心肌梗死不同时间段基质细胞衍生因子-1(SDF-1)的含量与左心功能变化的相互关系.方法 23只中华小型猪随机分为实验组及对照组,实验组开胸结扎冠状动脉左前降支制备急性心肌梗死模型并依据梗死后不同时间点随机分为6个亚组,分别行超声心动图检查,测量左室舒张末期内径(LVDd)、左室射血分数(LVEF)和左室短轴缩短率(LVFS),应用二维斑点追踪技术测量左室整体圆周应变(GCS)及整体径向应变(GRS).各组实验终点动物处死后取相应心肌梗死组织应用实时荧光定量PCR法测定SDF-1含量.结果与对照组相比,各实验组心肌组织内SDF-1含量均明显升高,LVEF、LVFS、GCS和GRS均降低,LVDd明显增大.心肌组织内SDF-1含量和左室短轴乳头肌水平GCS有一定相关性(r=-0.580,P=0.000).结论心肌组织内SDF-1的释放与GCS有一定相关关系.
목적 탐토심기경사불동시간단기질세포연생인자-1(SDF-1)적함량여좌심공능변화적상호관계.방법 23지중화소형저수궤분위실험조급대조조,실험조개흉결찰관상동맥좌전강지제비급성심기경사모형병의거경사후불동시간점수궤분위6개아조,분별행초성심동도검사,측량좌실서장말기내경(LVDd)、좌실사혈분수(LVEF)화좌실단축축단솔(LVFS),응용이유반점추종기술측량좌실정체원주응변(GCS)급정체경향응변(GRS).각조실험종점동물처사후취상응심기경사조직응용실시형광정량PCR법측정SDF-1함량.결과 여대조조상비,각실험조심기조직내SDF-1함량균명현승고,LVEF、LVFS、GCS화GRS균강저,LVDd명현증대.심기조직내SDF-1함량화좌실단축유두기수평GCS유일정상관성(r=-0.580,P=0.000).결론 심기조직내SDF-1적석방여GCS유일정상관관계.
Objective To investigate the relationship between the content of stromal cell-derived factor-1 (SDF-1) in myocardium in different period of myocardial infarction and left ventricular function.Methods Twenty three Chinese miniature pigs were randomly divided into the experimental group and the control group.The swines in experimental group were prepared as acute myocardial infarction model by ligating anterior descending coronary artery and were randomly divided into 6 subgroups according to the different time points after infarction.The left ventricular end-diastolic diameter (LVDd),left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) were measured respectively.Global circumferential strain (GCS) and global radial strain (GRS) of left ventricle were measured.The content of SDF-1 were also measured by real-time quantitative PCR.Results Compared with the control group,SDF-1 levels were significantly elevated,and LVEF,LVFS,GCS and GRS were reduced.However,LVDd were significantly increased.The content of SDF-1 and GCS has a negative correlation (r =-0.580,P =0.000).Conclusions The content of SDF-1 in myocardial tissue have a certain relationship with GCS of left ventricular myocardium.