中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2015年
9期
50-51
,共2页
格列齐特%成骨细胞%增殖%分化%凋亡
格列齊特%成骨細胞%增殖%分化%凋亡
격렬제특%성골세포%증식%분화%조망
Gliclazide%Osteoblast%Proliferation%Differentiation%Apoptosis
目的 探讨格列齐特对高糖浓度培养的成骨细胞MC3T3-E1增殖、分化及凋亡的影响.方法 16.5 mmol/L葡萄糖浓度的培养液体外培养MC3T3-E1,分为正常对照组和20 μmol/L格列齐特组,干预48 h,CCK-8法检测细胞增值率,流式细胞仪检测细胞凋亡率,荧光定量PCR法检测成骨细胞分化标志物骨桥蛋白(OPN).结果 与对照组相比,格列齐特组成骨细胞增殖率为24.30％.对照组和格列齐特组细胞早期凋亡率分别为14.57％、10.77％.与对照组相比,格列齐特组能促进OPN mRNA的表达(P＜0.05).结论 高糖环境中格列齐特能促进MC3T3-E1的增殖、分化,抑制其凋亡.
目的 探討格列齊特對高糖濃度培養的成骨細胞MC3T3-E1增殖、分化及凋亡的影響.方法 16.5 mmol/L葡萄糖濃度的培養液體外培養MC3T3-E1,分為正常對照組和20 μmol/L格列齊特組,榦預48 h,CCK-8法檢測細胞增值率,流式細胞儀檢測細胞凋亡率,熒光定量PCR法檢測成骨細胞分化標誌物骨橋蛋白(OPN).結果 與對照組相比,格列齊特組成骨細胞增殖率為24.30％.對照組和格列齊特組細胞早期凋亡率分彆為14.57％、10.77％.與對照組相比,格列齊特組能促進OPN mRNA的錶達(P＜0.05).結論 高糖環境中格列齊特能促進MC3T3-E1的增殖、分化,抑製其凋亡.
목적 탐토격렬제특대고당농도배양적성골세포MC3T3-E1증식、분화급조망적영향.방법 16.5 mmol/L포도당농도적배양액체외배양MC3T3-E1,분위정상대조조화20 μmol/L격렬제특조,간예48 h,CCK-8법검측세포증치솔,류식세포의검측세포조망솔,형광정량PCR법검측성골세포분화표지물골교단백(OPN).결과 여대조조상비,격렬제특조성골세포증식솔위24.30％.대조조화격렬제특조세포조기조망솔분별위14.57％、10.77％.여대조조상비,격렬제특조능촉진OPN mRNA적표체(P＜0.05).결론 고당배경중격렬제특능촉진MC3T3-E1적증식、분화,억제기조망.
Objective To investigate the effects of gliclazide on proliferation,differentiation and apoptosis of the MC3T3-E1 osteoblast exposed to high glucose condition.Methods The MC3T3-E1 cultured in vitro high glucose condition(16.5 mmol/L)were divided into two groups,control group and gliclazide administration group(20 μmol/L),and were exposed for 48 hours.Cell proliferation was tested with CCK-8.Cell apoptosis was detected by quantitative flow cytometry.The mRNA expressions of OPN was detected by semi-quantitative RT-PCR.Results Compared with control group,the proliferation rate of gliclazide group was 24.30％.In control group and gliclazide group,the apoptotic rates were 14.57％ and 10.77％.Compared with control group,gliclazide group stimulated OPN mRNA expression(P ＜0.05).Conclusions Gliclazide can improve the proliferation,differentiation and inhibit apoptosis of MC3T3-E1 in hyperglycemia condition.
