CAJ | 학술논문

用不同浓度的胰岛素培养人肾小管上皮细胞株48 h后,采用实时定量PCR检测人尿酸盐转运子mRNA的相对表达量.随胰岛素浓度的增加,人尿酸盐转运子mRNA表达水平明显降低(5、25、125、500 μIU/ml时的相对表达量分别为0.95、0.40、0.24、0.23).提示离体培养条件下的肾小管上皮细胞人尿酸盐转运子基因表达改变与胰岛素浓度的变化有关.
용불동농도적이도소배양인신소관상피세포주48 h후,채용실시정량PCR검측인뇨산염전운자mRNA적상대표체량.수이도소농도적증가,인뇨산염전운자mRNA표체수평명현강저(5、25、125、500 μIU/ml시적상대표체량분별위0.95、0.40、0.24、0.23).제시리체배양조건하적신소관상피세포인뇨산염전운자기인표체개변여이도소농도적변화유관.
Human proximal tubule epithelial cell line,HK-2 cells,were cultured with various concentrations of insulin for 48 h.Human urate transporter (hUAT) mRNA was detected by realtime quantitative PCR.hUAT mRNA levels were down-regulated by insulin (5,25,125,500 μIU/ml)in a dose-dependent manner (relative expression median were 0.95,0.40,0.24,and 0.23).In vitro,the expression of hUAT mRNA in HK-2 cells is associated with the concentration of insulin.