CAJ | 학술논문

目的了解云南省大理地区HCV基因分型及主要流行基因型6n和3a HCV E基因遗传进化和分子特征.方法 2014年1-5月在大理州人民医院收集临床诊断丙型肝炎患者血清;HCVRNA提取,RT-PCR扩增和序列分析.结果收集到HCV患者血清24份,21份HCV 5UTR基因扩增阳性,其中HCV基因3型15例(3a4例,3b 11例)和HCV基因6型6例(6k 4例,6n 2例).2株HCV(10和4)E1/E2基因序列分析结果显示:4与3a型HCV同源性最高,87.98％±3.07和87.97％±2.82,10病毒与6n型HCV同源性最高,90.30±1.87和90.54±1.53;2株病毒E1/E2基因均存在10个潜在糖基化位点;与其他病毒相比,2株病毒仅有5个T细胞抗原表位预测分值发生变化,其余37个抗原表位预测分值变化不大.结论大理地区目前以基因3型(3a、3b)和基因6型(6k、6n)为主要流行HCV基因型或基因亚型,大理地区流行的3a和6n型HCV的致病性、免疫学等生物学特性未发生明显变化.
목적 료해운남성대리지구HCV기인분형급주요류행기인형6n화3a HCV E기인유전진화화분자특정.방법 2014년1-5월재대리주인민의원수집림상진단병형간염환자혈청;HCVRNA제취,RT-PCR확증화서렬분석.결과 수집도HCV환자혈청24빈,21빈HCV 5UTR기인확증양성,기중HCV기인3형15례(3a4례,3b 11례)화HCV기인6형6례(6k 4례,6n 2례).2주HCV(10화4)E1/E2기인서렬분석결과현시:4여3a형HCV동원성최고,87.98％±3.07화87.97％±2.82,10병독여6n형HCV동원성최고,90.30±1.87화90.54±1.53;2주병독E1/E2기인균존재10개잠재당기화위점;여기타병독상비,2주병독부유5개T세포항원표위예측분치발생변화,기여37개항원표위예측분치변화불대.결론 대리지구목전이기인3형(3a、3b)화기인6형(6k、6n)위주요류행HCV기인형혹기인아형,대리지구류행적3a화6n형HCV적치병성、면역학등생물학특성미발생명현변화.
Objective To understand the genotypes of hepatitis C virus(HCV) and the genetic evolution and molecular characteristics of the predominant genotypes (6n and 3a) currently circulating in the Dali,Yunnan province.Methods From January to May 2014,sera were collected from patients with HCV infection in the First People' s Hospital of Dali.RNA was extracted from the serum samples,which were analyzed using RT-PCR with 5'UTR and E1/E2 primers.The RNA was sequenced,and sequence analysis was performed using bioinformatics software.Results Of 24 serum specimens from patients suspected of infection with HCV,21 were confirmed positive for HCV by RT-PCR with HCV 5UTRspecific primer,of which 15 were HCV genotype 3 (4 cases of 3a and 11 cases of 3b) and 6 were HCV genotype 6 (4 cases of 6k and 2 cases of6n).The homology of the nucleotide sequence and amino acid sequence of E1/E2 gene between isolate 4 and genotype 3a were up to 87.98％ ± 3.07and 87.97％ ± 2.82.The homology of the nucleotide sequence and aminoacid sequence between isolate 10 and genotype 6n were up to 90.30 ± 1.87 and 90.54 ± 1.53.There were 10 potential glycosylation sites on both of E1/E2 gene of two isolates HCV isolates.Only the predicted value of 5 T cell epitopes of 4 and 10 increasedor decreased because of amino acid mutations.The rest of predicted value of epitope slightly changed.Conclusion HCV genotype 3 (3a and 3b) and genotype 6 (6k and 6n) were the predoniuant geuotypes or subtypes currently circulating in the Dali,Yunnan province.The virulence and immunological characteristics of HCV 3a and 6n did not change significantly.