中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2015年
2期
124-126
,共3页
鲁伟峰%艾军红%刘亚丽%刘春艳%谢正德
魯偉峰%艾軍紅%劉亞麗%劉春豔%謝正德
로위봉%애군홍%류아려%류춘염%사정덕
疱疹病毒4型,人%基因型
皰疹病毒4型,人%基因型
포진병독4형,인%기인형
Herpesvirus 4,human%Genotype
目的 分析儿童IM、EBV-HLH、CAEBV中EBV EBER基因特征,探讨EBV变异株与EBV感染疾病临床表型间的相关性.方法 应用PCR方法扩增288例IM、52例EBV-HLH和9例CAEBV全血标本的EBNA3C和EBER基因,根据EBNA3C扩增产物的大小进行EBV分型;EBER基因扩增产物经测序后应用BioEdit 7.0.9和Mega4.0.2软件进行分析.统计分析不同EBV亚型和EBER基因型与临床表型的关系.结果 349例标本EBNA3C基因扩增阳性,EBV-Ⅰ/II型的检出率分别为98.6％和1.4％,共检测到EB-6m、EB-8m和EB-7m等3种EBER基因型,其检出率分别为96％、2.9％和1.1％,不同EBER基因型在IM、EBV-HLH、CAEBV中的检出率差异无统计学意义.结论 儿童EBV感染疾病中EBV以EBV-Ⅰ型为主,EB-6m基因型是EBER基因型中的优势基因型.
目的 分析兒童IM、EBV-HLH、CAEBV中EBV EBER基因特徵,探討EBV變異株與EBV感染疾病臨床錶型間的相關性.方法 應用PCR方法擴增288例IM、52例EBV-HLH和9例CAEBV全血標本的EBNA3C和EBER基因,根據EBNA3C擴增產物的大小進行EBV分型;EBER基因擴增產物經測序後應用BioEdit 7.0.9和Mega4.0.2軟件進行分析.統計分析不同EBV亞型和EBER基因型與臨床錶型的關繫.結果 349例標本EBNA3C基因擴增暘性,EBV-Ⅰ/II型的檢齣率分彆為98.6％和1.4％,共檢測到EB-6m、EB-8m和EB-7m等3種EBER基因型,其檢齣率分彆為96％、2.9％和1.1％,不同EBER基因型在IM、EBV-HLH、CAEBV中的檢齣率差異無統計學意義.結論 兒童EBV感染疾病中EBV以EBV-Ⅰ型為主,EB-6m基因型是EBER基因型中的優勢基因型.
목적 분석인동IM、EBV-HLH、CAEBV중EBV EBER기인특정,탐토EBV변이주여EBV감염질병림상표형간적상관성.방법 응용PCR방법확증288례IM、52례EBV-HLH화9례CAEBV전혈표본적EBNA3C화EBER기인,근거EBNA3C확증산물적대소진행EBV분형;EBER기인확증산물경측서후응용BioEdit 7.0.9화Mega4.0.2연건진행분석.통계분석불동EBV아형화EBER기인형여림상표형적관계.결과 349례표본EBNA3C기인확증양성,EBV-Ⅰ/II형적검출솔분별위98.6％화1.4％,공검측도EB-6m、EB-8m화EB-7m등3충EBER기인형,기검출솔분별위96％、2.9％화1.1％,불동EBER기인형재IM、EBV-HLH、CAEBV중적검출솔차이무통계학의의.결론 인동EBV감염질병중EBV이EBV-Ⅰ형위주,EB-6m기인형시EBER기인형중적우세기인형.
Objective To analyze the characteristic of EBER gene of Epstein—Barr virus in EBV infection in children in Beijing area.Methods Total DNA was extracted from whole blood of 288 patents with EBV-associated infectious mononucleosis (IM),52 patients with EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH) and 9 patients with chronic active Epstein-Barr virus (CAEBV) infection in Beijing Children' s Hospital.The subtype of EBV type Ⅰ / Ⅱ was defined by the size of amplicon of the EBNA3C genes.The amplified EBER gene products were sequenced directly and the sequences were analyzed by BioEdit 7.0.9 and MEGA 4.0.2.The relationship between EBV subtype or EBER genotype and clinical phenotype were statistically analyzed.Results Type Ⅰ EBV was present in 98.6％ samples (344/ 349) and type Ⅱ EBV in 1.4％ samples (5/349).Three EBER genotypes,including EB-6m,EB-8m and EB-7m,were defined in this study.Thc detection rates of EB-6m,EB-8m and EB-7m were 96％,2.9％ and 1.1％ respectively.There were no significant differences in the frequencies of the three genotypes among IM,EBV-HLH and CAEBV (P ＞ 0.05).Conclusion EBV type Ⅰ is predominant in EBV infection in children in Beijing area.EB-6m genotype was predominant genotype in this area.
