中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
16期
1226-1229
,共4页
蔡春林%姚相杰%卓菲%何雅青%杨贵清
蔡春林%姚相傑%卓菲%何雅青%楊貴清
채춘림%요상걸%탁비%하아청%양귀청
柯萨奇病毒A组4型%疱疹性咽峡炎%VP1区%基因型
柯薩奇病毒A組4型%皰疹性嚥峽炎%VP1區%基因型
가살기병독A조4형%포진성인협염%VP1구%기인형
Coxsackievirus A4%Herpangina%VP1 Sequence%Genotype
目的 分析从2012年和2014年深圳市2起疱疹性咽峡炎疫情中获得的6株柯萨奇病毒A组4型(CVA4)的VP1区遗传进化特征.方法 采用荧光定量反转录(RT)-PCR法对疱疹性咽峡炎患儿咽拭子标本进行总肠道病毒(EV)、人肠道病毒71型(EV71)、柯萨奇病毒A组16型(CVA16)、CVA4、CVA6和CVA10进行检测,对CVA4阳性样本采用RT-PCR方法扩增其VP1区全长序列,并进行核苷酸序列测定和系统进化分析.结果 在2起疫情中共鉴定出6株CVA4病毒,均为GIb基因亚型.其中的2014年深圳CVA4病毒株与2012年深圳CVA4病毒株核苷酸的同源性仅为94.1％(变异率达5.9％);氨基酸的同源性为98.3％,共发现5个氨基酸突变aa22N-S、aa34T-A、aa63N-S、aa165A-D、aa200T-A.进化分析表明,2012年深圳CVA4病毒株与山东省KF150144株进化关系最近;而2014年深圳CVA4病毒株与吉林省JQ715709株的进化关系最近.结论 2014年深圳CVA4病毒株和2012年病毒株的VP1区存在较大的核苷酸变异,基因内部走势明显不同.
目的 分析從2012年和2014年深圳市2起皰疹性嚥峽炎疫情中穫得的6株柯薩奇病毒A組4型(CVA4)的VP1區遺傳進化特徵.方法 採用熒光定量反轉錄(RT)-PCR法對皰疹性嚥峽炎患兒嚥拭子標本進行總腸道病毒(EV)、人腸道病毒71型(EV71)、柯薩奇病毒A組16型(CVA16)、CVA4、CVA6和CVA10進行檢測,對CVA4暘性樣本採用RT-PCR方法擴增其VP1區全長序列,併進行覈苷痠序列測定和繫統進化分析.結果 在2起疫情中共鑒定齣6株CVA4病毒,均為GIb基因亞型.其中的2014年深圳CVA4病毒株與2012年深圳CVA4病毒株覈苷痠的同源性僅為94.1％(變異率達5.9％);氨基痠的同源性為98.3％,共髮現5箇氨基痠突變aa22N-S、aa34T-A、aa63N-S、aa165A-D、aa200T-A.進化分析錶明,2012年深圳CVA4病毒株與山東省KF150144株進化關繫最近;而2014年深圳CVA4病毒株與吉林省JQ715709株的進化關繫最近.結論 2014年深圳CVA4病毒株和2012年病毒株的VP1區存在較大的覈苷痠變異,基因內部走勢明顯不同.
목적 분석종2012년화2014년심수시2기포진성인협염역정중획득적6주가살기병독A조4형(CVA4)적VP1구유전진화특정.방법 채용형광정량반전록(RT)-PCR법대포진성인협염환인인식자표본진행총장도병독(EV)、인장도병독71형(EV71)、가살기병독A조16형(CVA16)、CVA4、CVA6화CVA10진행검측,대CVA4양성양본채용RT-PCR방법확증기VP1구전장서렬,병진행핵감산서렬측정화계통진화분석.결과 재2기역정중공감정출6주CVA4병독,균위GIb기인아형.기중적2014년심수CVA4병독주여2012년심수CVA4병독주핵감산적동원성부위94.1％(변이솔체5.9％);안기산적동원성위98.3％,공발현5개안기산돌변aa22N-S、aa34T-A、aa63N-S、aa165A-D、aa200T-A.진화분석표명,2012년심수CVA4병독주여산동성KF150144주진화관계최근;이2014년심수CVA4병독주여길림성JQ715709주적진화관계최근.결론 2014년심수CVA4병독주화2012년병독주적VP1구존재교대적핵감산변이,기인내부주세명현불동.
Objective To analyze the phylogeny of the VP1 region of Coxsackie virus A4 (CVA4) from herpangina cases of Shenzhen in 2012 and 2014.Methods Real-time reverse transcription(RT)-PCR method was used to test virus such as human enterovirus71,coxsackievirus A16,coxsackievirus A4,coxsackievirus A6 and coxsackievirus A10.The VP1 gene of CVA4 positive samples were amplified by RTPCR and sequenced.Then the homology and phylogeny analysis of the CVA4 VP1 region was performed.Results The six CVA4 isolates identified in the herpangina cases during 2012 and 2014 were mostly closed with GIb genotypes.The nucleotide and amino acid homology between them were 94.1％ (nucleotide mutation rate was 5.9％) and 98.3％,five amino acid mutation were found in CVA4 strain 2014 of Shenzhen:aa22N-S,aa34T-A,aa63N-S,aa165A-D,aa200T-A.The phylogenetic analysis based on VP1 region demonstrates that CVA4 strain of Shenzhen in 2012 had the nearest genetic relationship with CVA4 strain of Shandong isolated in 2010(KF150144).However,CVA4 strain of Shenzhen in 2014 had the nearest genetic relationship with CVA4 strain of Jilin (JQ715709)isolated in 2006.Conclusions It reveals that all CVA4 strains from the two outbreak of herpangina belong to genotype GIb,the degree of variation in VP1 region of CVA4 strain of Shenzhen in 2014 is obvious compared with that in 2012.There is an obvious difference on internal trend of evolution lineage between the CVA4 strains from 2012 and 2014.
